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Extraction of genomic DNA from blood, cells and animal tissues
Catalog number / packaging
Mat. No | Ref. No | No. of preps |
CDP304-02 | 50 preps | |
CDP304-03 | 200 preps |
- StorageRoom temperature (15-25℃)
- DescriptionThe TIANamp Genomic DNA Kit provides a fast and easy silica-based format for DNA purification. The kit simplifies the purification of DNA from a wide range of sample types, including animal blood (≤ 1 ml), cultured cells, and tissues commonly encountered in life science, veterinary, and genotyping applications. Most samples could be directly lysed with the provided buffer system, eliminating the need for mechanical disruption and also reducing hands-on time. The kit purifies high-quality DNA without proteins and inhibitors. The purified DNA is suitable for PCR, restriction digestion and Southern blot.
- Required ReagentsRed Cell Lysis Buffer (for blood samples more than 200 μl, Cat# 4992810), ethanol, RNase A (optional)
- Features■ Silica-membrane technology provides fast way to purify ready-to-use high quality gDNA.■ High DNA yield, excellent repeatability.■ This kit significantly removes contaminants and inhibitors, facilitating downstream applications.
- Important Notes■ Please use fresh samples to ensure the integrity of the purified gDNA.■ If the sample starting amount exceeds the recommended range of the kit, it is recommended to increase the amount of Buffer GA and Buffer GB, and split the column loading in separate times, or perform the experiment using two spin columns.■ For long-term storage of the gDNA, it is recommended to store in buffer TE. Please ensure that the OD260/OD280 value is in the range of 1.7-1.9, and the concentration of gDNA is 100-300 ng/μl.
- DNA Extraction Yield From Various Plant Tissues
Publication
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Journals
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- Dual-responsive nanocarriers for efficient cytosolic protein delivery and CRISPR-Cas9 gene therapy of inflammatory skin disordersScience Advances2024-04-19 Read Article.. 2 days after delivery by a TIANamp Genomic DNA Kit (TIANGEN) according to the standard protocols.
- Gadd45g insufficiency drives the pathogenesis of myeloproliferative neoplasmsNature Communications2024-04-06 Read ArticleTotal genomic DNA from cell lines was extracted using TIANamp Genomic DNA kit (DP304; TIANGEN; Beijing; China).
- A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virusVeterinary Research2024-04-04 Read ArticleASFV genomic DNA was extracted from cell culture supernatant and cells using the TIANamp Genomic DNA Kit (Tiangen Biotech; Beijing; China).
- Genomic evidence for human-mediated introgressive hybridization and selection in the developed breedBMC Genomics2024-04-02 Read ArticleGenomic DNA was isolated from these samples with the use of the TIANamp Genomic DNA Kit (TIANGEN; Beijing; China).
- Enterohemorrhagic Escherichia coli effector EspF triggers oxidative DNA lesions in intestinal epithelial cellsInfection and Immunity2024-04-01 Read ArticleGenomic DNA was extracted from cell pellets using the TIANamp Genomic DNA kit (DP304) (Tiangen) according to the manufacturer??s instructions.
- TIANamp Genomic DNA Kit
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- Go2biotech
- E-mail: maggie@go2biotech.com / morgan@go2biotech.com
- Telephone:+86 755 8399 5017
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