TIANamp FFPE DNA Kit

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High-efficient purification of DNA from formalin-fixed, paraffin-embedded tissues by xylene treatment

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• StorageRoom temperature (15-25℃)
• DescriptionThe TIANamp FFPE DNA Kit applies xylene deparaffinization method to remove paraffin, and provides special lysis condition to extract DNA from formalin-fixed and paraffin-embedding (FFPE) samples. This method overcomes the inhibitory effect of formalin cross-linking. By combining the specific columns and a special buffer system, high-quality DNA can be extracted into a small elution volume. The purified DNA has good genome integrity, high purity and stable and reliable quality, which is ideally suitable for diagnosis and academic research.
• Required ReagentsXylene, ethanol, RNaseA (optional)
• Features■ Wide application: The kit could be used to extract gDNA from various FFPE samples.■ Easy extraction procedure:  Easy extraction and purification of high-quality, high-yield and ready-to-use DNA with good repeatability.■ Stable and reliable: Complete removal of contaminants and PCR inhibitors.
• Applications■ PCR and RT-qPCR■ SNP and STR gene analysis■ Pharmacogenomics research

Publication

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• Autophagy mediated FTH1 degradation activates gasdermin E dependent pyroptosis contributing to diquat induced kidney injury.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association2023-12-19 Read ArticleAbstract: Acute kidney injury (AKI) induced by diquat (DQ) progresses rapidly; leading to high mortality; and there is no specific antidote for this chemical.. Our limited knowledge of the pathogenic toxicological mechanisms of DQ has hindered the development of treatments against DQ poisoning.. Pyroptosis is a form of programmed cell death and was recently identified as a novel molecular mechanism of drug-induced AKI.
• Human Parvovirus B19 May Be a Risk Factor in Myasthenia Gravis with ThymomaAnnals of Surgical Oncology2022-12-12 Read Articlee sections obtained from representative paraffin blocks were deparaffinized in xylene and rehydrated in a graded ethanol series. Subsequently; genomic DNA was extracted using the TIANamp FFPE DNA Kit (Tiangen; DP331; China) in accordance with the manufacturer??s instructions and stored at ?????20 ??C until use. Nested PCR was performed to amplify a region of the B19V genome as previously described. 15 F
• ASCL2 Maintains Stemness Phenotype through ATG9B and Sensitizes Gliomas to Autophagy InhibitorAdvanced Science2022-07-26 Read Articledependently made by two experienced pathologists according to the World Health Organization (WHO) classification (2016). The DNA of glioma samples was obtained with a TIANamp FFPE DNA Kit (DP331) from Tiangen BIotech Co. Ltd.; IDH1/2 mutation analysis was performed by an ABI 3500DX Genetic Analyser with an IDH1/2 mutation detection kit from Yuanqi Bio Co. Ltd.; MGMT methylation status was determine
• Genetic features of TP53 mutation and its downstream FOXA1 in prostate cancer.Bioscience trends2022-07-22 Read Article.. was extracted using a paraffin-embedded tissue DNA extraction kit (Tiangen Biochemical Technology Co.; Ltd.; DP331-02)
• Direct administration of mesenchymal stem cell??derived mitochondria improves cardiac function after infarction via ameliorating endothelial senescenceBioengineering & Translational Medicine2022-07-02 Read Articleerase dUTP nick end labeling (TUNEL) assay (Life Technology; #C10625) was utilized to measure apoptosis in vivo according to the manufacturer’s instructions. DNA was isolated using DNA isolation kit (Tiangen; #DP331). Quantification was performed by qPCR with SYBR green??based detection (Thermo Fisher Scientific) as previously described. 14 Briefly; relative mitochondria DNA: nuclear DNA (mtDNA:n
• TIANamp FFPE DNA Kit – GDP331-02
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