morgan@go2biotech.comTGuide Smart Envir-DNA Kit-Tiangen OSE- ( Go2biotech.com – Distribution for Reagents and instruments )
Protocol
- Prefilled single sample cartridge
1) Take out a prefilled single sample cartridge and invert it to re-suspend the
magnetic beads; Gently shake to concentrate the reagent and magnetic beads to
the bottom of the cartridge. Before use, remove the sealing film carefully to avoid
liquid spatter or spills.
2)Add the Buffer TB/Buffer TE of appropriate volume (60~100 μl) into the 5th well of
the single sample reagent cartridge. - Suggested dosage for different samples
1)For common soil or fecal samples: Take 250 mg of soil samples (loam, clay,
subsoil, garden soil, farmland soil, etc.) and 100 mg of fecal samples (e.g.,
cattle and sheep feces, poultry feces, poultry intestinal contents, fish and shrimp
intestinal contents, etc.) in Envir-DNA Grinding Beads Tubes; for fecal samples
preserved in alcoholic preservation solution, take an appropriate amount of the
sample (not more than 100 mg of the dry mass) by high-speed centrifugation
to remove the preservation solution. For fecal samples containing alcohol
preservative, take an appropriate amount of sample (dry matter mass not more
than 100 mg) and centrifuge it at high speed to remove the preservative, then
transfer it to the Envir-DNA Grinding Beads Tube.
Note: If the soil or fecal sample is high in impurities and rich in microorganisms,
reduce the sample input appropriately. Otherwise, it may result in varying degrees
of color residue in the eluate.
2)Samples with high water content: e.g. fermentation broth, cellar yellow water,
washed honey or nectar, milk, etc., take an appropriate volume of sample,
centrifuge at 12,000 rpm (~13,400×g) for 1-2 min, remove the supernatant, and
collect the precipitate amount of about 10-150 mg, or when there is no significant
precipitation after centrifugation, 50-100μl of liquid can be retained in the aspirate
when discarding the supernatant and then add the 600μl Buffer SNA, resuspend
and mix well, then transfer to Envir-DNA Grinding Beads Tube and proceed to the
next step.
3)Aqueous or Air Filter Membrane: Cut up the filter membrane, 47 mm diameter filter
membrane is cut up and put into 2 ml Envir-DNA Grinding Beads Tube, 147 mm
diameter filter membrane is cut up and can be dispensed into 2-3 tubes of 2 ml
Envir-DNA Grinding Beads Tubes or 1 tube of 5ml Grinding Beads Tube.
Note: Only 48×2 ml Envir-DNA Grinding Beads Tubes are included in the kit, if
more 2 ml Envir-DNA Grinding Beads Tubes or 5 ml Grinding Tubes are needed,
the customer needs to bring their own.
4) Highly absorbent samples: e.g., powdered dacquoise, etc. Weigh 100 mg of
sample in Envir-DNA Grinding Beads Tubes.
5) Other special samples: - a) Honey: take 10-15 g of honey, add 3-4 times the volume of pure water (provided
- by the customer), mix well, centrifuge at 8,000 rpm (~6,000×g ) for 10 min,
- remove the supernatant, add 1ml of pure water (provided by the customer)
- to resuspend the precipitate, centrifuge at 8,000 rpm (~6,000×g ) for 10 min,
- remove the supernatant, and then add 600 μl of Buffer SNA, resuspend the
- precipitate and transfer to Envir-DNA Grinding Beads Tube for the next step.
- b) Fruiting body of fungal, tissue samples, intestinal contents containing intestinal
- tissues: take 10-100 mg samples and cut them into Envir-DNA Grinding Beads
- Tube.
- Sample pre-treatment
1) Weigh the sample into the Envir-DNA Grinding Beads Tube according to the
procedure “2. Suggestions for different sample amounts”.
2)Add 600-700 µl of Buffer SNA and 10 µl of RNase A (10 mg/ml), vortex and mix to
thoroughly break up and resuspend solid samples in solution, homogenize with
TGrinder H24 and homogenize with a program of 20 sec of oscillation at 6 m/s
with 20 sec intervals for a total of 6 cycles.
TGuide Smart Envir-DNA Kit
All trademarks or registered trademarks appearing on this website are the property of their respective owners
This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
Need more info ? Contact us anytime. We’re here:
Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
Address: Rm 4L, Golden Century Building, Shennan Av. 6033#, Shenzhen, China
