RNAprep pure FFPE Kit – ( Tiangen Gdp439 )( Go2biotech.com – Distribution for Reagents and instruments )

For purification of RNA from formalin-fixed, paraffin-embedded tissues

RNAprep pure FFPE Kit – ( Tiangen Gdp439 ) Catalog number / packaging

Description
The RNAprep Pure FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions can remove formaldehyde modifications of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase I and Buffer RDD for optimized removal of genomic DNA contamination. The purified RNA can be used in various of downstream applications such as RT-PCR.

Required Reagents

Xylene, ethanol

Features
■ Silica membrane based technology to ensure high-purity of RNA.

■ Fast and simple process compared with conventional methods.

■ Suitable for downstream RT-PCR or RT-qPCR applications.

Applications
This kit is suitable for the purification of total RNA from formalin-fixed, paraffin-embedded tissue sections (FFPE).

Storage
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.

Other reagents could be store at room temperature (15-25℃)

RNAprep pure FFPE Kit – ( Tiangen Gdp439 ) Publication

• IGF-1R mediates crosstalk between nasopharyngeal carcinoma cells and osteoclasts and promotes tumor bone metastasisJournal of Experimental & Clinical Cancer Research : CR2024-02-12 Read Article.. paraffin sections was extracted using the RNAprep Pure FFPE Kit (TIANGEN) according to the manufacturer’s instructions for
• Comprehensive analysis of miRNA-mRNA regulatory pairs associated with colorectal cancer and the role in tumor immunity.BMC genomics2023-12-04 Read ArticleTotal RNA was extracted from FFPE tissues using RNAprep Pure FFPE Kit (TIANGEN) according to the manufacturer??s instructions.
• Comprehensive analysis of miRNA-mRNA regulatory pairs associated with colorectal cancer and the role in tumor immunityBMC Genomics2023-11-30 Read ArticleTotal RNA was extracted from FFPE tissues using RNAprep Pure FFPE Kit (TIANGEN) according to the manufacturer??s instructions.
• Construct dysregulated miRNA-mRNA interaction networks to conjecture possible pathogenesis for Stomach adenocarcinomasCancer Biomarkers2023-11-23 Read ArticleAbstract: BACKGROUND: Post-transcriptional regulation of mRNA induced by microRNA is known crucial in tumor occurrence; progression; and metastasis.. This study aims at identifying significant miRNA-mRNA axes for stomach adenocarcinomas (STAD).. METHOD: RNA expression profiles were collected from The Cancer Genome Atlas (TCGA) and GEO database for screening differently expressed RNAs and miRNAs (DE-miRNAs/DE-mRNAs).

RNAprep pure FFPE Kit – ( Tiangen Gdp439 )  Manual

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RNAprep pure FFPE Kit – ( Tiangen Gdp439 )

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