OSR-M202-TGuide Virus DNA RNA Kit

TGuide Virus DNA/RNA Kit

TGuide Virus DNA/RNA Kit
Cat. No. OSR-M202

Storage Conditions:

1. It can be stored dry at room temperature (15-30°C) for 12 months.
2. Carrier RNA lyophilized powder can be stored at room temperature until
   the expiration date. Carrier RNA is first dissolved in RNase-free ddH2O,
   and the Carrier RNA solution should be frozen and stored at -30~-15°C.

Product Description:
TGuide Virus DNA/RNA Kit is specially designed for use with TGuide M16
Automated Nucleic Acid Extractor. It is able to separate and purify highquality viral DNA/RNA from serum, plasma, lymph, cell-free body fluid, cell
culture supernatant, urine or various virus preservation solutions. Plastic
consumables used in the kit are treated with DNase/RNase-free treatment,
and each sample runs independently. The system well avoids various
possibilities of cross-contamination between samples. The kit is able to
extract viral DNA or RNA from 200 µl or 400 µl samples and is economical
and convenient to integrate and use.
Virus nucleic acid obtained by magnetic bead separation technology is
suitable for high sensitivity PCR and quantitative PCR detection. The kit
has been verified by downstream detection of HBV, HCV, HIV and influenza
viruses.

Product Features:
Simple and fast: Ultrapure virus nucleic acids can be obtained in 57/66 min
from 200 μl/400 μl sample.
No contamination: Independent sealed prepacked reagent cartridges can
avoid the possibility of cross-contamination..
Safe and harmless:The kit and the operation process do not need to use
organic solvents harmful to human body such as phenol and chloroform.

Note: Read this note before using this kit.

1. This kit must be combined with TGuide M16 Automatic Nucleic Acid Extractor.
2. Repeated freezing and thawing of the sample should be avoided, otherwise the
   extraction yield will be decreased.

Preparation of Carrier RNA Solution
Add 310 μl of RNase-free ddH2O to a tube filled with 310 μg of Carrier RNA
lyophilized powder, and completely dissolve carrier RNA to obtain a solution with
a final concentration of 1 μg/μl. Aliquot the solution into RNase-free centrifuge
tubes according to experimental conditions and store at -30~-15°C. When in
use, take out the corresponding solution according to the times of extraction.
The solution should avoid repeated freezing and thawing, and the freezing and
thawing times should not exceed 3 times.

Operation steps:

1. Add 200 μl /400 μl of virus preservation solution such as serum, plasma or
   lymph to the sample tube, and add 20 μl /40 μl of protease K and 6 μl /12 μl of
   Carrier RNA solution.
2. Place the sample tube in the well 4 of the T-rack. Run the program No.202 (virus
   nucleic acid extraction program) and select the corresponding sample volume
   and final elution volume.
   Note: When operating according to the above steps, it is recommended to
   select an elution volume of 60 µl to obtain a higher elution concentration.

OSR-M202-TGuide Virus DNA RNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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