OSR-M104-TGuide Large Volume Blood Genomic DNA Kit (1.2 ml)

TGuide Large Volume Blood Genomic
DNA Kit (1.2 ml)
Cat. No. OSR-M104

Storage Conditions:
It can be stored dry at room temperature (15-30°C) for 12 months.

Product Description:
TGuide Large Volume Blood Genomic DNA Kit is specially designed to
cooperate with TGuide M16 Automated Nucleic Acid Extractor to extract
DNA (including genomic DNA, mitochondrial DNA, viral DNA) from
1.2 ml whole blood sample. Reagents needed for cell lysis and protein
degradation, magnetic beads specifically adsorbing DNA, washing buffer
and the like are prepacked in the reagent cartridges, and purified DNA is
eluted in a low-salt buffer solution. The length of genomic DNA extracted
by the kit is 20-30 kb, suitable for PCR or other enzymatic reactions.
Genomic DNA isolated from whole blood samples by magnetic bead
separation technology can be directly used in various conventional
operations without purification, including enzyme digestion, PCR, library
construction, Southern hybridization and other experiments.

Product Features:
Simple and fast: Ultrapure genomic DNA can be obtained with only 76 min.
Reliable results: The obtained DNA is free from protein and RNA
contamination and is able to use for PCR or fluorescence quantitative PCR.
Safe and harmless: The kit and the operation process do not need to use
organic solvents harmful to human body such as phenol and chloroform.y
such as phenol and chloroform.

Note: Read this note before using this kit.

1. This kit must be combined with TGuide M16 Automatic Nucleic Acid
   Extractor.
2. Repeated freezing and thawing of the sample should be avoided,
   otherwise the extraction yield will be decreased

Operation steps:

1. Add 1.2 ml of blood sample to the sample tube, and add 40 µl of
   Protease K.
2. Place the sample tube in the well 4 of the T-rack. Run program No.104
   (large volume whole blood genomic DNA extraction program) and only
   select the final elution volume.
   Note: It is recommended to select an elution volume of 200 µl or 300
   µl when operating according to the above steps, as the sample volume
   is relatively large and a higher elution concentration can be obtained
   under this elution volume.

OSR-M104-TGuide Large Volume Blood Genomic DNA Kit (1.2 ml)

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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