NR102-TIANSeq Fast RNA Library Kit (illumina)

TIANSeq Fast RNA Library Kit (illumina)
Cat. no. 4992375/4992376

Storage Conditions
The kit should be stored at -30~-15°C. Avoid repeated freezing and
thawing. The shelf life is one year.

Product Description
The TIANSeq Fast RNA Library Kit (Illumina) is a non-directional
transcriptome library construction kit specialized developed for the
Illumina high-throughput sequencing platform. The kit adopts a rapid onetube protocol for rapid library construction of RNA samples. After doublestranded cDNA synthesis, the end-repair and dA-tailing can be completed in one step. The resulting product can be directly used for the adapter
ligation without purification. In addition, the kit provides a specially
designed high-efficiency high-fidelity polymerase, which ensures the
obtained PCR-enriched product with high fidelity and no base preference.
The suitable input samples are total RNA with rRNA removed (containing
mRNA and other non-coding RNAs), or mRNA isolated directly from total
RNA. The input amount of the total RNA sample is 10 ng-1 μg; and the
input amount of the mRNA sample can be as low as 500 pg.
Applications: Suitable for the construction of RNA library for Illumina highthroughput sequencing platform.
Sample input amount: 10 ng-1 μg of total RNA; For animal, plant and
fungal mRNA, as low as 500 pg.

Other Recommended Reagents

1. TIANSeq Single-Index Adapter (Illumina) (Cat#4992641/4992642/4992378)
2. TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359/4992979)

Product Highlights

1. This kit can be used to perform transcriptome analysis on mRNA and noncoding RNAs (rRNA excluded), such as lncRNA.
2. The operation process is simple and can be used for rapid library
   construction of RNA samples.
3. High library conversion rate. Suitable for efficient library conversion of low
   input samples.
   4.The fidelity of PCR amplification is high with no base preference.

Precautions Please carefully read these precautions before using
this kit.

1. Attention should be paid in the operating process to avoid crosscontamination between nucleic acid samples and products.
2. Please use RNase- or DNase-free pipette tips and EP tubes for the
   experiments.
3. Before starting, wipe down work area with RNase and DNase cleaning
   reagents such as RNase Away (Molecular BioProducts, Inc). Make sure
   there is no contamination of RNase and DNase.
4. Please read the instructions carefully before the experiment. The
   experiment can be paused at certain steps and samples can be stored
   according to the institutions.
5. Use high-quality RNA samples with RIN value ≥ 7.0 and good integrity
   for rRNA removal or mRNA isolation, otherwise the quality of the RNA
   library will be affected.

Protocol
I. RNA Fragmentation and Priming

1. Preparation:
   (1) Take out the total RNA with rRNA removed or mRNA samples from the
   -80°C freezer and place on ice to slowly thaw the samples.
   (2) Before starting the experiment, make sure the input amount of the
   total RNA with rRNA removed or mRNA is within 1-100 ng.
   Note: It is important to determine the input amount of the total RNA
   with rRNA removed or mRNA. It is recommended to use Agilent 2100
   to analyze the quality and concentration of the samples. The rRNA
   residue should be controlled within 10% to avoid its effect on the data
   analysis quality after library construction.
2. Procedures
   Take out the Frag/1st Strand Buffer from the -20°C fridge and thaw on
   ice. Mix by votexting, and set up the following reaction in a PCR tube.
   Gently pipette up and down for 10 times to mix thoroughly. Place the
   sample tube in the thermal cycler and choose the reaction conditions
   for fragmentation according to the sizes of the insert

https://en.tiangen.com/upload/file/20220510/20220510162904_54939.pdf

NR102-TIANSeq Fast RNA Library Kit (illumina)

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