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High-efficiency miRNA extraction kit specifically targeting serum/plasma samples

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• StorageThe extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.
• DescriptionmiRcute Serum/Plasma miRNA Isolation Kit is a new generation of product specially developed for miRNA extraction from serum and plasma samples. The Buffer MZA in the kit has been well developed and improved, which has lysis capability and extraction sensitivity that are not available in common lysis solutions. The adsorption column in the kit adopts special silica matrix membrane filler, which greatly enhances its adsorption capability to RNA, especially small RNA(<200 nt). The extracted miRNA has better purity and higher quality, and is suitable for routine experiments such as downstream fluorescence quantification.
• Features■ High specificity: Specially designed for extracting miRNA in serum/plasma with optimized lysis buffer.■ High efficiency: High quality miRNA can be obtained within 1 h with high extraction yield.■ Ultra-pure: Column purification to avoid gDNA and salt contamination.
• ApplicationsThe extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.

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• Study of serum exosome miRNA as a biomarker for early onset adult ouclar myastthenia gravis.Gene2024-01-12 Read ArticleAbstract: Background: By extracting and sequencing miRNAs from serum exosomes of patients with early-onset ocular myasthenia gravis (OMG); generalized myasthenia gravis (GMG) and healthy controls; we screened differentially expressed miRNAs and explored the possibility as potential biomarkers for early-onset OMG.. Methods: Peripheral blood samples were collected from patients with early-onset OMG; early-onset GMG; and age-matched healthy subjects; with 6 samples in each group.. All these patients were diagnosed as MG for the first time and did not undergo any treatment.
• The plasma derived exosomal miRNA-483-5p/502-5p serve as potential MCI biomarkers in aging.Experimental gerontology2024-01-10 Read Article.. in exosome were extracted using the miRcute miRNA Isolation Kit (TIANGEN; DP503) according to the manufacturer’s instruction
• The Role of Cystathionine-??-Synthase, H 2 S, and miRNA-377 in Hypoxic-Ischemic Encephalopathy: Insights from Human and Animal Studies.Journal of molecular neuroscience : MN2024-01-01 Read ArticleAbstract: We aimed to investigate the mechanism underlying the roles of miRNA-377; Cystathionine-??-synthase (CBS); and hydrogen sulfide (H2S) in the development of hypoxic-ischemic encephalopathy (HIE).. We investigated the relationship between CBS; H2S; and miR-377 in both humans with HIE and animals with hypoxic-ischemic insult.. An animal model of fetal rats with hypoxic-ischemic brain injury was established; and the fetal rats were randomly assigned to control and hypoxic-ischemic groups for 15 min (mild) and 30 min (moderate) groups.
• Pulmonary tuberculosis biomarker miR-215-5p inhibits autophagosome-lysosome fusion in macrophages.Tuberculosis (Edinburgh, Scotland)2023-12-04 Read ArticleAbstract: The normal autophagy flux is beneficial for the rapid elimination of phagocytic pathogens by macrophages.. However; Mycobacterium tuberculosis interferes with the autophagy flux of macrophages to weaken their immune function and evade immune surveillance.. In this study; we found that miRNA-215-5p was downregulated in tuberculosis patients.
• Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1??2Biology2023-11-24 Read Article.. BALB/c mice were extracted using a miRcute Serum/Plasma miRNA Isolation Kit (TIANGEN; Shanghai; China) according to the manufacturer??s

miRcute Serum/Plasma miRNA Isolation Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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