MD113-1kb plus DNA Ladder

1 kb Plus DNA Ladder
Cat. no. 4992800/4992801
Storage: -30~-15°C for 2 years. For frequently use, it
can be stored at 2-8°C for 3 months.
Concentration: About 0.1 mg DNA/ml

Description
The 1 kb Plus DNA Ladder consists of twelve doublestranded DNA bands that are suitable as molecular weight standards for agarose gel electrophoresis.
Since this product is premixed with 1× Loading Buffer, it is convenient to load 3~6 μl of this product to agarose gel directly.
The ladder is composed of twelve individual DNA fragments (in base pairs): 10000, 8000, 5000, 2000,
1600, 1000, 7000, 500, 400, 300, 200 and 100. It contains
one intense band (1000 bp) for easy orientation. To load 6 μl of the product will provide 50 ng per DNA fragment with one intense fragment of 100 ng.

Buffer Contents
10 mM Tris-HCl (pH 8.4)
10 mM EDTA
0.02% Bromophenol Blue
5% Glycerol

Protocol

1. Directly load 3~6 μl of this product to the agarose
   gel (load 1μl product per 1 mm gel lane. The loading
   volume can be increased appropriately for wide
   lanes).
2. Electrophoresis condition: 1.0% agarose gel, 4~10 v/cm voltage.
3. DNA marker can be visualized using ethidium bromide under UV light.

Recommendations

1. Directly use, do not require heating.
2. For ideal results, please use new agarose gel and change electrophoresis buffer in time.