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Ultra-sensitive lncRNA specific reverse transcription reagent

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• StorageStore at -20°C
• DescriptionThe lnRcute lncRNA First-Strand cDNA Kit is a product specially developed for reverse transcription of long-chain non-coding RNA (lncRNA). Compared with mRNA, lncRNA has the characteristics of low abundance, large difference in GC content and more complex secondary structure, and traditional reverse transcription reagents are difficult to achieve ideal effects on lncRNA. This kit contains gDNase for efficiently removing genomic DNA, which can effectively avoid interference of residual genomic DNA on subsequent detection results. The reverse transcriptase used in lnR RT Enzyme Mix in this kit is FastKing RT Enzyme. This enzyme is a new molecular modified reverse transcriptase, with a hydrophobic motif added, and has stronger RNA affinity and thermal stability, which makes this kit outstanding in aspects of stress resistance and high reverse transcription efficiency for templates with GC content, complex secondary structure or low abundance. This kit is especially suitable for reverse transcription reaction of lncRNA with relatively low expression level and relatively complex secondary structure.
• Features■ High efficiency: Stable and efficient King reverse transcriptase with RT efficiency over 95%.■ Sensitivity: The sensitivity-enhancing components are added to the buffer solution, ensuring the high sensitivity with lower limit of detection reaching 0.1 ng total RNA.■ Stress resistance: Suitable for complex templates, perfect resistance to foreign interference■ Fast reaction: gDNA-free cDNA can be synthesized within 21 min
• Applications■ The reverse transcribed cDNA can be used for real time PCR of lncRNA, and for regular PCR of mRNA, real time PCR, cDNA library construction and other experiments.

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• AMPK/MTOR/TP53 Signaling Pathway Regulation by Calcitonin Gene-Related Peptide Reduces Oxygen-Induced Lung Damage in Neonatal Rats through Autophagy Promotion.Inflammation2024-03-19 Read ArticleAbstract: Our previous studies indicated that calcitonin gene-related peptide (CGRP) alleviates hyperoxia-induced lung injury and suggested the possible involvement of autophagy in this process.. Herein; we aimed to further explore the potential involvement of tumor protein p53 (TP53) and autophagy in the mode of action of CGRP against hyperoxia-induced lung injury in vitro and in vivo.. The study conducted tests on type II alveolar epithelial cells (AECII) and rats that were subjected to hyperoxia treatment or combined treatment of hyperoxia with CGRP; CGRP inhibitor; rapamycin (an autophagy agonist); 3-methyladenine (3-MA; an autophagy inhibitor); TP53 silencing/inhibitor (pifithrin-??); or expression vector/activator (PRIMA-1 (2;2-bis(hydroxymethyl)3-quinuclidinone)) and their corresponding controls.
• A long noncoding RNA functions in pumpkin fruit development through S-adenosyl-L-methionine synthetase.Plant physiology2024-02-28 Read Article.. instructions and then reverse-transcribed with the lnRcute lncRNA First-Strand cDNA Synthesis Kit (Tiangen; China).
• Transcriptomic profiling of long non-coding RNAs and messenger RNAs in the liver of mice during Toxoplasma gondii infection.Parasites & vectors2024-01-18 Read Article.. (Takara; Tokyo; Japan) and the lnRcute lncRNA First-Strand cDNA synthesis kit (TianGen; Beijing; China) were employed; respectively.
• SiRNF8 Delivered by DNA Framework Nucleic Acid Effectively Sensitizes Chemotherapy in Colon CancerInternational Journal of Nanomedicine2024-01-06 Read Article.. synthesis was performed using the FastKing cDNA first-strand Synthesis Kit from Tiangen; and further real-time quantitative PCR
• Integrative analysis of long noncoding RNAs dysregulation and synapse-associated ceRNA regulatory axes in autism.Translational psychiatry2023-12-11 Read Article.. (TransGen; Beijing; China); and lnRcute lncRNA First Strand cDNA Synthesis Kit (Tiangen; KR202-02; Beijing; China). qRT-PCR

lnRcute lncRNA First-Strand cDNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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