KG204-Blood Direct PCR Kit

Blood Direct PCR Kit
——For direct PCR analysis of blood without DNA purification

Blood Direct PCR Kit
Cat. No. 4992529/4992530

Storage
Blood Direct PCR Kit can be stored at -30~-15°C for up to 12 months. 2×Blood Direct PCR MasterMix needs to be thawed and mixed by gentle inverting before use. For regular use, this kit can be stored at 2-8°C for up to 3 months. Please avoid repeated freezing and thawing.

Introduction
Blood Direct PCR Kit allows direct PCR analysis of blood sample, without
the need of DNA purification and sample preparation. The PCR MasterMix
supplied in this kit is a 2 × premix, with which only the addition of blood
template and primers are required to start a PCR analysis. The PCR premix
in this kit contains Taq DNA polymerase which is genetically modified
towards specific stress resistance, and the PCR reaction system is also
optimized according to this stress resistant enzyme. Blood samples which
are stored by different methods can be used as templates to perform
PCR using this kit. The 3’ end of PCR product is dA, which allows the PCR
product to be directly used in TA cloning.
This kit can efficiently amplify single copy gene in human genomic DNA,
and could be widely used in research studies like genomic DNA fragment
amplification (≤5 kb), high throughput genetic analysis and genotyping (like
gene deletion).

Features
Simple and fast:DNA purification and sample preparation are not
necessary, which makes the whole PCR process quick and convenient.
High inhibiter resistance:DNA polymerase supplied in this kit is genetically
modified according to specific stress resistance, and the unique optimized
buffer system can also strengthen its inhibiter resistance.
High compatibility: This kit can be used to amplify DNA fragment with high
GC content and complicated secondary structure, and the length of DNA
fragment can be up to 5 kb.
Wide applicability: This kit can be used to analyze blood samples from
different species and under different storage conditions. (e.g. blood sample
from human, mouse, rat or poultry; blood stored at 4°C or frozen blood;
anti-coagulation blood with EDTA, citrate or heparin; liquefied blood clot
and dry blood spots on whatman 903® or FTA® card.)
High throughput:This kit can be used with 96/384-well PCR plates for
large-scale samples identification by PCR.
No contamination: Blood can be directly used as template without the
need for pretreatment, so cross-contamination can be avoided.

Important Notes Before Starting

1. Excessive blood templates inhibit the PCR amplification. Please refer to
   “Recommended volume of blood template” to adjust the dosage of templates.
   In a 20 μl reaction system, the optimum template volume is 1-2 μl. In the initial
   experiment, it is recommended to set the gradient template dosage at the
   range of 1-5 μl to determine the optimum template dosage.
2. If using other reaction system, please decrease or increase the template volume
   proportionally.
3. Due to the denaturation of hemoglobin in the blood template, the amplified
   product might present turbidity, which does not affect the subsequent
   electrophoresis detection. However, further purification is needed for
   subsequent experiments such as cloning and sequencing.

Protocol
Note: The following protocol is just for case study, please setup the reaction
condition according to specific circumstance (e.g. different structure of
primers and template).

1. Use human anti-coagulation blood as template, perform a PCR reaction by
   using Blood Direct PCR Kit to amplify a 1,000 bp DNA fragment.
2. Setup the PCR reaction according to the following table.

KG204-Blood Direct PCR Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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