morgan@go2biotech.comKG202-GMO Crop Extraction & Amplification Kit
GMO Crop Extraction & Amplification Kit (Part A)
GMO Crop Extraction & Amplification Kit(Part A)
Cat. No. 4992890
Storage Conditions
The kit can be stored for 12 months under dry conditions at room temperature
(15-30°C).
Introduction
This product is specially developed for the PCR detection of GMO crop.
The kit includes two parts: A and B. Part A of the kit provides reagents for
extracting GMO crop genomic DNA, while Part B provides reagents for PCR
detection of GMO crop DNA.
The unique lysis buffer contained in Part A of the kit can specifically and
fully lyse the tissues of five main crops—wheat, corn, rice, cotton and
soybean to release nucleic acid, protein and other related components.
Subsequent RNA, protein, metal ions and other impurities can be removed
to the maximum extent through specific RNase and phenol/chloroform
extraction to obtain genomic DNA with good purity and high yield for
subsequent PCR detection.
Features
Wide applicability: High-quality genomic DNA can be extracted from five
major GMO crops.
Easy and fast: The extraction of GMO crop genomic DNA can be completed
within 2 hours with no need for a large-scale freezing centrifuge. The
experiment has low requirements on instruments and equipment, thus
being suitable for research institutions at all levels to carry out rapid
genomic DNA extraction on GMO crops.
Important Notes Before Starting
- Avoid repeated freezing and thawing of the sample, otherwise the
extracted DNA fragments will be smaller and the extraction yield will be
decreased. - It is suggested to use fresh leaves for extraction to acheive optimal yield
and purity of genomic DNA. - All centrifugation steps are performed at room temperature using a
benchtop centrifuge. - Before using Buffer PL, please add mercaptoethanol to the final
concentration of 0.1%(V/V)
Protocol
- Take about 100 mg of fresh plant tissue or about 30 mg of dry weight
tissue, and grind with liquid nitrogen. - Quickly transfer the ground powder to a centrifuge tube with 700 μl
of 65°Cpreheated Buffer PL (add mercaptoethanol to the preheated
Buffer PL to make its final concentration 0.1% before the experiment).
After quickly inverting and mixing, place the centrifuge tube in a 65°C
water bath for 20 min. During the water bath, invert the centrifuge tube
to mix the samples several times. - Take out the centrifuge tube, and gently flick the tube to collect the
liquid drops on the tube cover and tube wall to the bottom. Add 6 μl of
RNase A (100 mg/mL), mix well, and incubate at room temperature for
10 min. - Add equal volume of phenol: chloroform: isoamyl alcohol (25:24:1), mix
thoroughly and centrifuge at 12,000 rpm (~13,400×g) for 5 min.
Note: For plant tissues rich in polyphenol or starch, the equal volume
of phenol: chloroform (1:1) can be used for a second time extraction. - Carefully transfer the upper aqueous phase obtained in the previous
step into a new centrifuge tube, add equal volume of isopropanol, and
mix thoroughly. - Centrifuge at 12,000 rpm(~13,400×g) for 5 min.
- Discard the supernatant, add 500 µl 70% ethanol and mix well.
- Centrifuge at 12,000 rpm(~13,400×g) for 5 min.
- Repeat steps 7 and 8.
10.Invert the centrifuge tube on clean absorbent paper until the ethanol
volatilizes completely. Make sure the precipitation remain in the
centrifuge tube.
Note: The residue of ethanol will affect the subsequent enzyme
reaction (enzyme digestion, PCR, etc.). However, over-drying of DNA
precipitation should be avoided, for it will be difficult to dissolve the
DNA.
11.Add 100 µl Buffer TE to dissolve DNA and store under appropriate
conditions.
Note: If DNA is dissolved slowly, keep it in 65°Cwater bath for 10-30
min to accelerate the dissolution.
KG202-GMO Crop Extraction & Amplification Kit
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
Need more info ? Contact us anytime. We’re here: Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
