morgan@go2biotech.comGDP601-DE-TGuide Smart Blood Genomic DNA Kit
For genomic DNA purification from blood and buffy coat.
TECHNICAL MANUAL
Cat. no. GDP601-DE
Note: To use the TGuide Smart Blood Genomic DNA Kit, you must have the
TGuide Smart Blood Genomic DNA(program no. DP601-01) installed on
the TGuide S16/S32 pro Nucleic Acid Extractor.
Table Contents
Kit Contents
Blood Genomic DNA reagent composition
Storage condition
Product
Features
Notes
Operational steps
1.Preparation of blood DNA extraction reagent
2.Operation steps of TGuide S16 Nucleic Acid Extractor
Appendix
1.Program
2.Related Products
TGuide Smart Blood Genomic DNA Kit
Cat. no. GDP601-DE
The kit can be stored in dry conditions at room temperature (15~30°C) for 12months
Product
The kit adopts magnetic beads and a unique buffer system to isolate and purify
genomic DNA with high quality from blood. The uniquely embedded magnetic beads
have a strong affinity for nucleic acid under certain conditions. When the conditions
are changed, the magnetic beads can release the absorbed nucleic acid to rapidly
separate and purify it. The whole process does not involve organic reagents and
is safe and convenient. The extracted genomic DNA has large fragments and high
purity and is stable and reliable in quality. It can be used to perfectly fit with TGuide
S16 Nucleic Acid Extractor of TIANGEN for automated extraction.
DNA purified by this kit is suitable for downstream experiments including enzymatic
digestion, PCR, chip analysis, library construction and Southern Blot.
Features
- Simple and fast:It can be extracted automatically with TGuide S16 Nucleic Acid
Extractor, and ultra-pure genomic DNA can be obtained within 1 hr. - Wide use: It is suitable for frozen or fresh whole blood and buffy coat.
- High purity:The DNA obtained has high purity and can be directly used for PCR,
enzymatic digestion, hybridization and other experiments
Notes
- The sample should avoid repeated freezing and thawing, otherwise the DNA
fragments extracted will be small and the amount of extraction will be reduced. - The pre-processing methods of different samples will be slightly different. We
recommend that the buffy coat samples should be mixed with a vortex mixer
before loading. If blood samples have cell clusters, users also need to mix them
with a vortex mixer before loading. - After sample pre-processing, take an appropriate amount of the sample and add it
to the 1st well of the single sample reagent cartridge. - The elution buffer (Buffer TB) is not prefilled, so users need to add the Buffer TB
to the 5th well of the single sample reagent cartridge before performing on the
instrument. The recommended range of Buffer TB is 60~100 µl. The smaller the
elution volume, the higher the nucleic acid concentration, but the lower the total
yield may be. On the contrary, the larger the elution volume, the lower the nucleic
acid concentration, and the higher the total yield may be. Users can adjust it in the
range of 60~100 µl as needed
Operational steps
- Preparation of blood DNA extraction reagent
1.1 Take out a prefilled single sample reagent cartridge and invert it to re-suspend
the magnetic beads; Gently shake to concentrate the reagent and magnetic
beads to the bottom of the cartridge. Before use, remove the sealing film
carefully to avoid liquid spatter or spills.
1.2 Add the Buffer TB of appropriate volume (60~100 µl) into the 5th well of the
single sample reagent cartridge. - Operation steps of TGuide S16 Nucleic Acid Extractor
Please read the following precautions before loading:
(1) The samples should be balanced to room temperature.
(2) For buffy coat samples, mix it with a vortex mixer for 2 min before adding to
the 1st well of the single sample reagent cartridge.
(3) If the blood samples have cell clusters, they can also be mixed with a vortex
mixer for 1~2 min before adding to the 1st well of the single sample reagent
cartridge.
(4) If the blood sample is anticoagulant blood of poultry, birds, amphibians or
lower organisms, their red blood cells have nucleus cells, and the sample
processing volume should be adjusted to 5~20 µl. PBS or normal saline (selfprovided) should be added to supplement to 200 µl.
2.1 Add 200~250 µl whole blood sample or 100 µl buffy coat and 20 µl Proteinase
K respectively into 1st well of the single sample reagent cartridge, and place
the cartridge on the reagent tank bracket of TGuide S16 Nucleic Acid Extractor.
2.2 Place the reagent tank bracket on the plate base in the TGuide S16 Nucleic
Acid Extractor. Insert the Tip Combs into the slots to ensure they are well
connected and firmed.
- Preparation of blood DNA extraction reagent
1.1 Take out a prefilled single sample reagent cartridge and invert it to re-suspend
the magnetic beads; Gently shake to concentrate the reagent and magnetic
beads to the bottom of the cartridge. Before use, remove the sealing film
carefully to avoid liquid spatter or spills.
1.2 Add the Buffer TB of appropriate volume (60~100 µl) into the 5th well of the
single sample reagent cartridge.
- Operation steps of TGuide S16 Nucleic Acid Extractor
Please read the following precautions before loading:
(1) The samples should be balanced to room temperature.
(2) For buffy coat samples, mix it with a vortex mixer for 2 min before adding to
the 1st well of the single sample reagent cartridge.
(3) If the blood samples have cell clusters, they can also be mixed with a vortex
mixer for 1~2 min before adding to the 1st well of the single sample reagent
cartridge.
(4) If the blood sample is anticoagulant blood of poultry, birds, amphibians or
lower organisms, their red blood cells have nucleus cells, and the sample
processing volume should be adjusted to 5~20 µl. PBS or normal saline (selfprovided) should be added to supplement to 200 µl.
2.1 Add 200~250 µl whole blood sample or 100 µl buffy coat and 20 µl Proteinase
K respectively into 1st well of the single sample reagent cartridge, and place
the cartridge on the reagent tank bracket of TGuide S16 Nucleic Acid Extractor.
2.2 Place the reagent tank bracket on the plate base in the TGuide S16 Nucleic
Acid Extractor. Insert the Tip Combs into the slots to ensure they are well
connected and firmed.
2.3 If you use the TGuide S16 Nucleic Acid Extractor, select the corresponding
program DP601-01 file on the touch screen, click the icon in the lower right
corner of the screen and click the “RUN” button at the bottom of the screen to
start the experiment.
2.4 At the end of the automated extraction process, take the DNA out of the 5th
well of the cartridge and store it under appropriate conditions. Single sample
reagent cartridge and tip comb are for single use only.
GDP601-DE-TGuide Smart Blood Genomic DNA Kit
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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