GDP452-RNA Easy Fast Plant Tissue Kit

RNA Easy Fast
Plant Tissue Kit

RNA Easy Fast Plant Tissue Kit
Cat. no. GDP452

Selected Reagent
DNase I (1500 U) (TIANGEN, Cat. no. GRT411)

Storage
The reagent is stored at room temperature (15-30°C) for 15 months. The
selected RNase-Free DNase I is stored at 2-8°C for 15 months.

Introduction
This product is a RNA easy fast plant tissue kit developed based on the
genomic DNA removal technology exclusively by TIANGEN. It does not need
toxic reagents such as β-mercaptoethanol or DTT, and is widely suitable for
rapid extraction of RNA from various plant samples. RNA extraction can be
completed within 30 minutes. The total RNA extracted by this kit has high
yield, good purity and no contamination of protein and other impurities. It
can be used in many downstream experiments such as RT-PCR, Real Time
RT-PCR, Chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro
translation, RNase protection analysis and molecular cloning, etc

Pay attention to the following aspects to prevent RNase
contamination:

1. Change new gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-Free plastic and tips to avoid cross contamination.
3. Although RNA will not be degraded by RNase once it is in lysis buffer SG,
   RNase-free plastics and glassware should be used for further treatment.
   To wipe off RNase, the glassware can be heated at 150°C for 4 hours,
   while plastic can be dipped in 0.5 M NaOH for 10 min, washed by RNaseFree ddH2
   O thoroughly, and sterilized.
4. Use RNase-Free ddH2
   O to prepare the solutions. (Add DEPC into water
   in clean glass container to a final concentration of 0.1% (v/v). Incubate
   overnight and autoclave for 15 min to remove any trace of DEPC.)

Important Notes

1. If the subsequent experiments require high RNA purity, DNase I (provided
   by customer) digestion can be selectively carried out according to Step 6.
2. Ethanol(96%-100%) should be added to the Buffer RW before the first
   use as indicated on the bottle.
3. The following operations are performed at room temperature unless
   specified.
   

Reagents need to be prepared by Customer
96-100% ethanol and DNase I (optional)

GDP452-RNA Easy Fast Plant Tissue Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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