GDP424-TRNzol Universal Reagent

TRNzol Universal Reagent
For isolation of high quality total RNA from cell and tissue samples

TRNzol Universal Reagent
Cat. no. GDP424

Introduction
TRNzol Universal Reagent is developed based on regular TRNzol Reagent
with the addition of indicator. It has better lysis ability and higher
sensitivity. It could be used to isolate total RNA from samples like virus,
bacteria, fungus, animal, plant tissue and body fluid. It maintains the
integrity of RNA while disrupting cells and dissolving cell components
during sample homogenization.
TRNzol Universal Reagent can be applied to isolate RNA from both small
amount of samples (50-100 mg tissue or 5 × 106
cells) and large amount
of samples (≥1 g tissue or ≥107
cells) of human, animal, plant or bacteria,
within one hour. DNA and protein contamination is eliminated thoroughly
from RNA products. Isolated RNA can be used in Northern Blot, Dot
Blot, poly(A) selection, in vitro translation, RNase protection assay and
molecular cloning.

Storage
TRNzol Universal Reagent should be stored protected from light at 2-8°C
for up to 15 months.

Important Notes

1. Before the addition of chloroform, homogenized samples in TRNzol
   Universal Reagent can be stored at –70°C for at least one month.
2. The RNA can be stored in 75% ethanol for at least 1 year at -20°C, or at
   least 1 week at 2-8°C.
   

Notes of preventing RNase contamination

1. Change gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-Free plastic and tips to avoid cross contamination.
3. RNA can be protected in TRNzol Universal Reagent. But RNA must be
   stored or processed in RNase-Free plastic or glassware. To wipe off
   RNase, the glassware can be heated at 150°C for 4 hours, while plastic
   can be dipped in 0.5 M NaOH for 10 min, washed by RNase-Free ddH2O
   thoroughly, and sterilized.
4. Use RNase-Free ddH2O to confect solution. (Add DEPC into water in
   clean glass container to a final concentration of 0.1% (v/v). Incubate
   overnight and autoclave for 15 min to remove any trace of DEPC.)

GDP424-TRNzol Universal Reagent

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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