morgan@go2biotech.comFP411-miRcute Plus miRNA qPCR Kit (SYBR Green)
miRcute Plus miRNA qPCR Kit (SYBR Green)
——For detection of miRNA using real-time RT-PCR (SYBR Green)
miRcute Plus miRNA qPCR Kit
(SYBR Green)
Cat. No. 4992887/4992779
Storage
The miRcute Plus miRNA qPCR Kit (SYBR Green) should be stored immediately
upon receipt at -30~-15°C, protected from light. Thaw each components and
mix thoroughly before use. If the2× miRcute Plus miRNA Premix (SYBR&ROX)
are thawed but not used, it is important to thoroughly mix them prior to refreezing (The layering of salts during the thawing process and subsequent
crystallization during freezing will damage the enzyme and decrease product
performance). This kit could be stored at -30~-15°C for 1 year.
Introduction
miRcute Plus miRNA qPCR Kit is designed for miRNA real-time PCR by using SYBR
Green I. The kit is composed of 2× miRcute plus miRNA Premix, 50× ROX Reference
Dye and Reverse Primer (10 µM).
2× miRcute plus miRNA premix (with SYBR and ROX) was developed specifically
for quantitative detection of miRNA. The DNA polymerase in this kit is the
antibody modified hot-start DNA polymerase. Combined with the unique buffer,
the hot-start DNA polymerase ensures high specificity, sensitivity and accurate
quantitative detection in a wide range.
Note: The kit has to be used with miRcute Plus miRNA First-Strand cDNA Kit (Cat.
no. 4992786/4992909).
Important Notes
- The kit contains SYBR Green I. Store and prepare PCR reaction in dark condition.
- Pipet the reaction solution and aliquot with sterile tips and consumable items
to avoid contamination.
Reagents and Materials Not Supplied
- RNase-free ddH2O
- Forward primer
Important Notes
- Follow the most common design principles of primers.
- Based on mature miRNA sequence, change U to T to design forward prime.
- Tm value of Reverse Primer (10µM) in the kit is about 65°C. Ensure that Tm
value of forward prime is basically consistent with supplied Reverse Primer (10
µM). - If Tm value of the designed forward prime is too low according to principle 2, it
is recommended to add several nucleotides (G or C is optional) to 5’-terminal,
or add one or several adenines to 3’-terminal. Optionally, modify both 5’
terminal and 3’ terminal at the same time - If Tm value of the designed forward prime is too high according to principle 2,
remove several nucleotides from 5’ or 3’ terminal.
FP411-miRcute Plus miRNA qPCR Kit (SYBR Green)
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