morgan@go2biotech.comFP209-Talent qPCR PreMix (SYBR Green)
Talent qPCR PreMix (SYBR Green)
——For fast, universal, quantitative, real-time PCR using SYBR Green
Talent qPCR PreMix (SYBR Green)
Cat. No. 4992882/4992775/4992866
Storage
The Talent qPCR PreMix (SYBR Green) Kit can be stored at -30~-15°C for
one year. Itshould be stored at -30~-15°Cimmediately after receipt,
protected from light.Thaw the 2×Talent qPCR PreMix (SYBR Green) and
50×ROX Reference Dye and mix thoroughly before use. If the 2×Talent
qPCR PreMix (SYBR Green) and 50×ROX Reference Dye are thawed but
not used, it is important to thoroughly mix them prior to refreezing.
The delamination of salts during the thawing process and subsequent
crystallization during freezing will damage the enzyme and decrease
product performance. For frequent use, 2×Talent qPCR PreMix (SYBR
Green) can be stored at 2-8°C for 3 months. Repeated freeze-thaw cycles
should be avoided.
Introduction
The Talent qPCR PreMix (SYBR Green) Kit is designed for SYBR Green I
based quantitative PCR assays, which enables fast and specific quantitative
results. Optimized premix could reduce the running time and is suitable for
regular and fast real-time PCR thermal cycler.
2×Talent qPCR PreMix (SYBR Green) adopts antibody modified Anti Taq
DNA polymerase. Combined with the unique PCR buffer, it could ensure
a sensitive PCR detection on any Real-Time PCR thermal cycler. Total
running time could be reduced by 50% compared to regular real-time PCR
program. In addition, the H-competitor factor and EP components added
in the PreMix make the product have good sample universality especially
for templates with complex secondary structures, with long PCR products
and with more PCR inhibitors. Meantime accurate quantification, high
amplification efficiency, high specificity and wide credibility range could be
achieved.
Important Notes
- The 2×Talent qPCR PreMix adopts anti-TaqDNA polymerase modified by
antibodies, which is combined with special fast qPCR Buffer system that
can greatly reduce denaturation, annealing and extension time, and
save up to 50% reaction time. - The H-Competitor factor is specially added in the PreMix, which could
compete the hydrogen bond with DNA, and could open the DNA double
strand easily, making the products have good sample universality,
especially for templates with complex secondary structures, with long
PCR products and with more PCR inhibitors. - The special fast PCR Buffer system of this product contains the unique
PCR stabilizer, EP, which can effectively protect the enzyme’s activity,
resist the interference of various PCR inhibitors, and ensure the high
amplification efficiency, high amplification sensitivity and wide range of
reliability of 2×Talent qPCR PreMix. - 2×Talent qPCR PreMix is premixed with SYBR Green I. When the PCR
reaction solution is prepared, the rapid real-time PCR reaction can be
carried out simply by adding template, primer and sterilized distilled
water. The operation is simple and convenient. - This product comes with ROX Reference Dye, which can eliminate signal
background and calibrate the fluorescent signal error between different
wells. It is convenient for the customer to choose the corresponding
concentration depend on different real-time PCR systems. - 2×Talent qPCR PreMix is packaged with colorless transparent tubes.
After detection, lighting will not affect the quantitative results of the
system.
Principle of Operation
This product uses antibody-modified hotstart DNA polymerase for rapid
PCR amplification, and the amplified PCR products are quantified by
detecting the fluorescence intensity of SYBR Green I in the reaction
process. It is suitable for regular and fast real-time PCR thermal cycler.
- Antibody modified hotstart Taqhas a short activation time, the
denaturation, annealing and elongation time can be greatly reduced
and meanwhile avoiding the amplification of nonspecific products. This
product can save up to 50% reaction time, and get experimental results
more quickly. - The H-competitor factor and optimized PreMix make the products
have good sample universality especially for templates with complex
secondary structures, with long PCR products and with more PCR
inhibitors. - In view of the differences in the structure of cDNA template and gDNA
template, this product is specially optimized for system reaction steps
of PCR, so that gDNA template which is difficult to be amplified can also
obtain good PCR results.
Important Notes
- Gently mix the reagents by inverting the tubes and centrifuge briefly
prior to use. Do NOT vortex and avoid producing bubble. - The purity of primers is important for the specificity of PCR. Primers
purified by PAGE or more superior methods are recommended. - Typically, best amplification results can be obtained using a primer
concentration of 0.3 μM. However, for individual determination of
optimal primer concentration, a primer titer from 0.2 μM to 0.5 μM can
be performed. - In a 20 μl reaction volume, the amount of cDNA template is usually less
than 100 ng, and genomic DNA is less than 50 ng. If reverse transcribed
products are used as template, the template input should be no more
than 20% of the total PCR reaction volume.
FP209-Talent qPCR PreMix (SYBR Green)
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
