morgan@go2biotech.comFP207-FastFire qPCR PreMix (SYBR Green)
FastFire qPCR PreMix (SYBR Green)
——For fast, quantitative, specific real-time PCR using SYBR Green
FastFire qPCR PreMix (SYBR Green)
Cat. no. 4992217/4992218/4992249
Storage
FastFire qPCR PreMix (SYBR Green) can be stored at -30~-15°C for one year.
It should be stored immediately upon receipt at -30~-15°C. 2 × FastFire
qPCR PreMix (SYBR Green I) and 50 × ROX Reference Dye should be thawed
and then mixed upside down gently to be homogenous before using. If the
Reagents have been thawed but not used, it is important to thoroughly
mix prior to re-freezing. (The layering of salts during the thawing process
and subsequent crystallization during freezing will damage the enzyme and
decrease product performance.) The reagents could be stored for up to 3
months at 2-8°C if frequently used. Please avoid refreezing and thawing
repeatedly.
Introduction
FastFire qPCR PreMix (SYBR Green) is designed for SYBR Green I based
quantitative PCR assays, enables fast and specific quantitative results.
Optimized premix could reduce the running time and is suitable for regular
and fast real-time PCR thermal cycler.
FastFire qPCR PreMix adopts antibody modified Anti Taq DNA polymerase.
Combined with the unique PCR buffer, it could ensure a sensitive PCR
detection on any Real-Time PCR thermal cycler. Total running time could be
reduced by 60% compared with regular real-time PCR program. Meantime
accurate quantification, high amplification efficiency, high specificity and
wide credibility range could be achieved.
Important Notes
- FastFire qPCR PreMix (SYBR Green) contains SYBR Green I. Store the
reagent in dark and avoid direct exposure to strong light during the
preparation of PCR reaction mixtures. - Gently mix the reagents by inverting the tubes and centrifuge briefly
prior to use. Do NOT vortex and avoid producing bubble. - The purity of primers is important for the specificity of PCR. Primers
purified by PAGE or more superior methods are recommended. - Typically, best amplification results can be obtained using a primer
concentration of 0.3 µM. However, for individual determination of
optimal primer concentration, a primer titer from 0.2 µM to 0.5 μM can
be performed. - In a 20 µl reaction volume, the amount of cDNA template is usually
less than 100 ng, and genomic DNA is less than 50 ng. The reverse
transcription product, if used as template, should not comprise more
than 20% of the total PCR reaction volume.
FP207-FastFire qPCR PreMix (SYBR Green)
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