FastKing RT Kit (With gDNase)-GKR116

.. 1849 / Publication

Efficiently read all kinds of sequences and accurately identify low abundance templates

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• Packing Size20 μl×25 rxn
• StorageStore at -20℃.
• DescriptionThe kit is an efficient, stable and fast reverse transcription system capable of removing genomic DNA contamination. The kit contains gDNase for efficiently removing genomic DNA, thus effectively avoiding interference of genomic DNA in total RNA. The high-efficiency reverse transcriptase FastKing RT Enzyme is a new type of modified reverse transcriptase, with a hydrophobic motif, which has stronger RNA affinity and thermal stability, thus further improving its reverse transcription efficiency and reaction rate, and making it more prominent in reading through RNA templates with high GC content and complex secondary structure and in stress resistance.
• Features■ High efficiency: The FastKing RT Enzyme is modified with hydrophobic motif, with RT efficiency more than 95%.■ Sensitive: As low as 1 ng templates can be accurately identified.■ Resistance: Capable of reverse transcription of complex templates, with perfect resistance to impurities.■ Fast procedure: gDNA-free cDNA can be synthesized efficiently within 21 min.
• ApplicationsThe reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction.

Publication

.. 1849 / Publication

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• Programmable RNA 5-methylcytosine (m5C) modification of cellular RNAs by dCasRx conjugated methyltransferase and demethylase.Nucleic acids research2024-04-15 Read Article.. cDNA synthesized using the FastKing RT Kit was amplified using 2 ?? Taq PCR Mix (TIANGEN Biotech; China) and 2 ??
• Programmable RNA 5-methylcytosine (m 5 C) modification of cellular RNAs by dCasRx conjugated methyltransferase and demethylaseNucleic Acids Research2024-04-12 Read Article.. was synthesized using the FastKing RT Kit (with gDNase) (TIANGEN Biotech; China) as per the
• microRNA-125b-1-3p mediates autophagy via the RRAGD/mTOR/ULK1 signaling pathway and mitigates atherosclerosis progression.Cellular signalling2024-04-09 Read Article.. the extracted RNA using the FastKing RT Kit (with gDNase; No. KR116; Tiangen Biotech; Beijing; China) following the manufacturer’s
• Assessing developmental neurotoxicity of emerging environmental chemicals using multiple in vitro models: A comparative analysis.Environmental pollution (Barking, Essex : 1987)2024-04-08 Read ArticleAbstract: Newly synthesized chemicals are being introduced into the environment without undergoing proper toxicological evaluation; particularly in terms of their effects on the vulnerable neurodevelopment.. Thus; it is important to carefully assess the developmental neurotoxicity of these novel environmental contaminants using methods that are closely relevant to human physiology.. This study comparatively evaluated the potential developmental neurotoxicity of 19 prevalent environmental chemicals including neonicotinoids (NEOs); organophosphate esters (OPEs); and synthetic phenolic antioxidants (SPAs) at environment-relevant doses (100 nM and 1 ??M); using three commonly employed in vitro neurotoxicity models: human neural stem cells (NSCs); as well as the SK-N-SH and PC12 cell lines.
• Therapeutic effect of notoginseng saponins before and after fermentation on blood deficiency ratsExperimental and Therapeutic Medicine2024-04-01 Read Article.. was reverse transcribed into cDNA using the FastKing RT Kit (Tiangen Biotech Co.; Ltd.) according to the manufacturer’s protocol. qPCR

FastKing RT Kit (With gDNase)

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