FastKing One Step RT-qPCR kit (SYBR Green）Tiangen GFP313 ( Go2biotech.com – Distribution for Reagents and instruments )

One-step Real-Time RT-PCR with SYBR-Green

FastKing One Step RT-qPCR kit (SYBR Green）Tiangen GFP313

Catalog number / packaging

 Manual

Packing Size
50 μl×50 rxn

Storage
Store in dark at -20℃.

Description
FastKing One Step RT-qPCR Kit is designed for Real-Time One Step RT-qPCR by using SYBR Green I. The kit can be used in real-time one step RT-qPCR of RNA targets, with reverse transcription and PCR taking place sequentially in the same tube. Therefore the operation is simple, and it minimizes the risk of contamination.

The 25× RT-PCR Enzyme Mix in this kit is a premix of TIANGEN’s novel reverse transcriptase (FastKing RTase), novel antibody modified hotstart Taq DNA polymerase and RNase Inhibitor. The FastKing RTase is a new type of molecular modified reverse transcriptase. With the specifically added hydrophobic motif, which has stronger RNA affinity and thermal stability. The FastKing RTase has improved reverse transcription efficiency and the ability to extend the RNA template with complex secondary structure. The new type of hotstart Taq DNA polymerase with excellent performance is used in the PCR process, making the PCR reaction after reverse transcription have higher amplification efficiency and specificity. In addition, the 2× FastKing RT-qPCR Buffer (SYBR Green) in this product is a new reaction system specially optimized for the above two key enzymes, including necessary ionic components, dNTPs, SYBR Green dye, one step stabilizer and enhancer, which can ensure that FastKing RTase and the new hotstart Taq DNA polymerase can play the most effective role in the whole one-step reaction process.

This kit provides good standard curves in a wide range of quantitative area. It can accurately and quantitatively detect a variety of high and low abundance target genes with good repeatability and high reliability.

Features
■ Increased reaction efficiency: The high-quality reverse transcriptase and Taq polymerse ensure the high reaction efficiency.
■ Easy and fast operation: The dual component form makes the procedure easy and fast.
■ Capable of Reading through complex templates: Suitable for RNA templates with high GC content and complex secondary structure.
■High compatibility: Compatible for RNA templates from different sample source or with impurities.

Applications
The RT-qPCR can be used to detect gene expression and RNA virus content in cells and tissues.

FastKing One Step RT-qPCR kit (SYBR Green）Tiangen GFP313 Publication

• Prenatal inflammation exposure accelerates lung cancer tumorigenesis in offspring mouse: possible links to IRE1??/XBP1-mediated M2-like polarization of TAMs and PD-L1 up-expressionCancer Immunology, Immunotherapy : CII2024-03-30 Read Article.. by One-step reverse transcription PCR kit (FP313-01; Tiangen; Beijing; China). ??-actin was regarded as
• Basic helix-loop-helix (bHLH) gene family in rye (Secale cereale L.): genome-wide identification, phylogeny, evolutionary expansion and expression analyses.BMC genomics2024-01-19 Read ArticleThe reverse transcription kit was selected from TianGen FastKing One-Step RT-qPCR Kit (SYBR; FP313-01).
• HOPX is a tumor-suppressive biomarker that corresponds to T cell infiltration in skin cutaneous melanomaCancer Cell International2023-06-21 Read Article.. In addition; the FastKing One Step RT??qPCR Kit (SYBR) (TIANGEN; China) and an Eppendorf
• Bortezomib inhibits ZIKV/DENV by interfering with viral polyprotein cleavage via the ERAD pathway.Cell chemical biology2023-05-22 Read Article.. Cat# AM1344 FastKing One Step RT-PCR Kit TIANGEN Cat# FP313-01 Experimental models: Cell lines Human: HEK293T cell line ATCC
• Theabrownin Isolated from Pu-Erh Tea Enhances the Innate Immune and Anti-Inflammatory Effects of RAW264.7 Macrophages via the TLR2/4-Mediated Signaling Pathway.Foods (Basel, Switzerland)2023-03-30 Read Article.. Kit (With gDNase) (KR116); and a FastKing One Step RT-qPCR SYBR Green Kit (FP313) were purchased from TianGen (Beijing;

FastKing One Step RT-qPCR kit (SYBR Green）Tiangen GFP313

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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