ET106-HotMaster Taq DNA Polymerase

HotMaster TaqDNA Polymerase
Cat. no. 4992771/4992772

Storage:-30~-15°C for two years.
Concentration: 2.5 U/µl

Introduction
HotMaster TaqDNA Polymerase is developed by TIANGEN to provide hot-start PCR for higher PCR
specificity. HotMaster inhibitor blocks the substrate binding site of HotMaster TaqDNA polymerases in a
temperature-dependent manner. Inactive polymeraseinhibitor complexes are formed at temperatures <40°C,where the affinity of HotMaster inhibitor for Hotmaster
TaqDNA polymerase is higher than the binding affinity of the template DNA. When the temperature increases to the specific annealing temperature of primers, the binding equilibrium shifts towards the complex formation with only target-specific primed template DNA. This minimizes the non-specific amplification in PCR and ensures high sensitivity and specificity. The PCR
process is fast and convenient. PCR products generated by HotMaster TaqDNA Polymerase have 3’-dA overhangs that can be directly used in TA-cloning.

Product Highlights

• HotMaster TaqDNA Polymerase does not need to be
  activated by high temperature incubation step.
• Continuous control of annealing temperature during PCR process.
• The length of PCR amplification target sequence can reach 5 kb.
• No protein contamination during PCR.
• The optimum extension temperature of the enzyme is 65°C, which can be adjusted from 60°C to 70°C.

Unit Definition
One unit of HotMaster TaqDNA Polymerase is defined as the amount that incorporates 10 nmol of dNTPs into acid-insoluble substrates within 30 min at 74°C with activated salmon sperm DNA as the template/primer.
Quality Control
Purity up to standard by SDS-PAGE assay; No activity of
exogenous nuclease is detected; Single gene in human
genome could be amplified effectively; No significant
activity change when stored at room temperature (15-30°C) for one week.

Application
Highly specific DNA amplification: suitable for highly sensitive amplification of genomic DNA with high
background (e.g. specific gene sites or detection of exogenetic virus in genomic DNA), DNA sequencing,
Multiplex PCR, TA-cloning etc.

ET106-HotMaster Taq DNA Polymerase

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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