morgan@go2biotech.comET104-Taq Platinum DNA Polymerase
TaqPlatinum DNA Polymerase
Cat. no.4992768
Storage: -30~-15°C for two years.
Concentration: 2.5 U/µl
Introduction
TaqPlatinum DNA Polymerase is a chemically modified hot-start polymerase with 3’-5’ and 5’-3’ exonuclease activity. It is inactive at ambient temperatures and must
be activated by heat treatment (5-10 min at 94°C). This prevents the extension of non-specifically annealed primers or primer–dimers at low temperatures during PCR setup, and therefor highly increases the sensitivity and specificity of PCR amplification. TaqPlatinum DNA Polymerase has unique high fidelity and it possesses higher extension rate and amplification efficiency than PfuDNA Polymerase.
TaqPlatinum DNA Polymerase generates PCR products with 3’-dA overhangs that can be directly used in TAcloning. To obtain higher cloning efficiency, however, PCR products could be purified and added 3’-dA overhangs before TA cloning procedures.
Unit Definition
One unit of TaqPlatinum DNA Polymerase is defined as the amount that incorporates 10 nmol of dNTPs into acid-insoluble substrates within 30 min at 74°C with activated salmon sperm DNA as the template/primer.
Quality Control
Purity up to standard by SDS-PAGE assay, and no exogenous enzyme activity should be included. Taq
Platinum DNA Polymerase needs to be capable to amplify single copy gene in human genome and need to be stable for 1 week under room temperature (15-30°C).
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT,
100 mM KCl, Stabilizers, 50% Glycerol
10×TaqPlatinum Buffer
BufferⅠ: 200 mM Tris-HCl (pH 8.8), 100 mM KCl,
100 mM (NH4)2SO4, 15 mM MgSO4, other components
BufferⅡ: 200 mM Tris-HCl (pH 9.0), 200 mM KCl,
60 mM (NH4)2SO4, 15 mM MgCl2, other components
● Please use Buffer Ⅰat first. When DNA amplification
cannot be confirmed, the result may be improved by
using Buffer Ⅱ.
Applications
Amplify DNA fragments from complex templates (e.g.Genome) with high fidelity, for applications such as gene cloning, Site-directed mutagenesis, SNP Analysis etc
ET104-Taq Platinum DNA Polymerase
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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