ET103-Long Taq DNA Polymerase

Long TaqDNA Polymerase

Cat. no. 4992766/4992767
Storage: -30~-15°C for two years.
Concentration: 2.5 U/µl

Introduction
Long TaqDNA Polymerase is an independently developed thermo-stable DNA polymerase with 3’-5’ exonuclease activity. It possesses high amplification efficiency and high fidelity. Provided with two kinds of buffer, Long Taq DNA Polymerase could amplify varied templates. For simple templates, it can amplify up to 40 kb; For complex templates such as GC-rich and repeated sequences, it
can syntherize up to 15 kb. The PCR products can be used directly in TA-cloning procedures. If required of high cloning efficiency, please purify, add A and then make T/A-cloning.

Unit Definition
One unit of Long TaqDNA Polymerase is defined as the amount that incorporates 10 nmol of dNTPs into acidinsoluble material within 30 min at 74°C with activated
salmon sperm DNA as the template-primer.

Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100mM KCl, Stabilizers, 50% Glycerol.

10× Long TaqBuffer
Provided with two kinds of buffer, 10×Long TaqBuffer I and 10×Long TaqBuffer II. Please use Buffer I first, if it fails to amplify the template, use Buffer II.

Applications
PCR amplification of long DNA fragments, and complex templates such as GC-rich and repeated sequences, e.g.,gene map construction, sequencing, molecular genetics research.

Quality Control
Test results show no activity of foreign nuclease.
Single copy gene in human genome could be amplified effectively. No significant activity change after storing at room temperature (15-30°C) for one week.

ET103-Long Taq DNA Polymerase

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