DP601-TGuide S32 Magnetic Blood DNA Kit Handbook 

TGuide S32 Magnetic Blood DNA Kit
Cat.no. 4992985

Storage
This kit can be stored at room temperature (15-30°C) under dry condition
for 12 months. If a precipitate has formed in Buffer under 2-8°C, please
place the buffer at room temperature or warm at 37°C for 10 min to
dissolve the precipitate.

Introduction
The kit adopts magnetic beads with unique separation function and a unique
buffer system to separate and purify high-quality genomic DNA from blood.
The unique embedded magnetic beads have strong affinity for nucleic acid
under certain conditions, and when the conditions change, the magnetic
beads release adsorbed nucleic acid, thus achieving the purpose of fast
separation and purification of nucleic acid.
The product is perfectly matched with TGuide S32 Automated Nucleic Acid
Extractor. Magnetic beads are adsorbed, transferred and released by special
magnetic rods, thus realizing the transfer of magnetic beads and nucleic
acids. The whole experimental process is safe and convenient. The extracted
genomic DNA fragments are large in size, with high purity and stable and
reliable quality.
The DNA purified by the kit is suitable for various conventional operations,
including enzyme digestion, PCR, library construction, Southern blot and other
experiments.

Features
Easy and fast:Ultrapure genomic DNA can be obtained within 48 min.
Ultrapure: The obtained DNA has high purity and can be directly used
in molecular biology experiments such as PCR, enzyme digestion,
hybridization, etc.

Protocol

  1. Preparation of Blood DNA extraction reagent
    Take out the vacuum package prepackaged 96-deep-well plate from
    the kit, mix it upside down for several times to resuspend the magnetic
    beads, remove the vacuum package. Gently shake the 96-deep well
    plate to concentrate the reagent and magnetic beads to the bottom of
    the 96-deep-well plate (or centrifuge at 500 rpm for 1 min by the plate
    centrifuge). Carefully tear off the aluminum foil sealing film before use
    to avoid vibration of the 96-deep-well plate and prevent liquid spillage.
  2. TGuide S32 Automated Nucleic Acid Extractor operation steps
    2.1 Add 200 μl of blood sample (the sample needs to be equilibrated
    to room temperature) and 10 μl Proteinase K into the 1st and 7th
    columns of the 96-well plate respectively. Place the 96-deep-well
    plate on the 96-deep-well plate base of TGuide S32 Automated
    Nucleic Acid Extractor.
    2.2 Insert the S32 Tip Combs into the slot of of magnetic rod tip comb
    slot of the TGuide S32 Extractor .
    2.3 Run the blood automatic extraction program on TGuide S32
    extractor

Turn on the supporting Windows Pad, double-click the “Purification” icon to enter the TGuide S32 control program, click “Run”, select the”*DP601-Blood” experimental program file and click the “Run” button in the lower right corner to start the experiment.

DP601-TGuide S32 Magnetic Blood DNA Kit Handbook 

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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