DP422-DNA RNA Isolation Kit

DNA/RNA Isolation Kit
For simultaneous purification of genomic
DNA and total RNA from the same animal cells or tissues

DNA/RNA Isolation Kit
(Spin Column)
Cat.no. 4992456

Storage
Buffer RLplus added with β-mercaptoethanol could stay 30 days at 2-8°C. All
the other reagents should be stored in dry place and at room temperature
(15-30°C) for 15 months.

Introduction
DNA/RNA Isolation Kit is designed for extracting both genomic DNA and
total RNA simultaneously from the same animal cells or tissue samples.
This kit is compatible with a wide range of animal cells and tissues. The
simple process allows the purification of high-quality DNA and RNA from
the same sample within 40-50 min. The purified DNA and RNA are eluted
separately and ready-to-use in downstream applications.

Notes of preventing RNase contamination

1. Wear gloves when handling RNA and all reagents, as skin is a common
   source of RNase. Change gloves frequently.
2. Use RNase-Free certified, disposable plastic ware and filter tips
   whenever possible.
3. Buffer RLplus could protect RNA. But for experiment, RNA should be
   stored or applied in RNase-Free plastic or glassware. To wipe off RNase,
   the glassware could be dried at 150°C for 4 hours, while plastics could
   be dipped in 0.5 M NaOH for 10 min, and washed by RNA-Free ddH2O
   thoroughly and sterilized.
4. Use RNase-Free ddH2O to prepare solution (RNase-Free ddH2O: add
   0.1 ml DEPC to 100 ml ddH2O and shake vigorously to bring DEPC into
   solution. Let the solution stand overnight. Autoclave to remove any
   trace of DEPC).

Important Notes

1. Add β-mercaptoethanol (β-ME) to Buffer RLplus to a final concentration
   of 1% before use. For example, add 10 μl β-mercaptoethanol (β-ME) per
   1 ml Buffer RLplus. Buffer RLplus may form precipitate during storage. If
   necessary, redissolve by warming it in 56°C, and then equilibrate to
   room temperature.
2. Before start, add ethanol (96-100%) to Buffer RW, Buffer PW and Buffer
   GD for a working solution, as described on the bottle.
3. The following operations were carried out at room temperature unless
   otherwise indicated.
4. For some sensitive RNA samples, genomic DNA may need to be removed
   completely in the f ollowing application. Please refer to DNase I digestion
   process with column.

DP422-DNA RNA Isolation Kit

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