morgan@go2biotech.comDP337-N96 DNAsecure Plant Kit
N96 DNAsecure Plant Kit
Fast and high throughput purification of high quality DNA from various fresh plant tissues
N96 DNAsecure Plant Kit
Cat. no. 4992718
(N96 Plate)
Storage
This kit can be stored for 15 months in a dry environment under room
temperature (15-30°C). If any precipitate forms in the buffers, it should be
dissolved by warming the buffers at 37°C before use
Introduction
This kit adopts a specific DNA binding spin column and unique buffer
system, which enables the extraction of genomic DNA from multiple
plant tissues. The silicon substrate material adopted in the N96 Plate
CB3 (H) is a new material of our company, which allows the column to
absorb DNA efficiently and specifically, and to remove protein as much
as possible. The genomic DNA extracted by this kit is integrate, pure and
stable.
The extracted gDNA can be used for various downstream experiments
including enzyme digestion, PCR, library construction and Southern
blot,etc.
Product Features
Simple and fast:Ultra-pure gDNA can be obtained within 1 h.
Wide sample types:It is applicable to various plant tissues.
Ultra-pure:The obtained gDNA with high purity can be used directly
in PCR, enzyme digestion, hybridization and other molecular biology
experiments.
Important Notes Please read the notes before using this kit.
- Avoid freezing and thawing of the sample, otherwise the DNA fragments
extracted will be smaller and the amount of extraction will be decreased. - Buffer LP1 may appear to be yellow, but it will not affect the extraction
efficiency. - If there’s precipitation in Buffer LP1 or LP2, please incubate it in 37°C
water bath and shake it before use. - All centrifugation steps should be carried out in a conventional table-top
microcentrifuge at room temperature (15-30°C).
Vacuum Protocol
The extraction steps 1-3 of the vacuum method are the same as the
centrifugation process.
- Connect the negative pressure vacuum device correctly and put
N96 Filtration Plate (H) on the device, and put a new N96 Well Plate
underneath it. Adjust the negative pressure to 40-70 kpa. Transfer the
centrifuged supernatant solution to N96 Filtration Plate (H). Turn on the
negative pressure device for 2 min to suction filtration. - Add Buffer LP3 of a volume 1.5 times to the filtrate into the filtrate on
N96 Well Plate (for instance, add 750 μl of Buffer LP3 into 500 μl of
filtrate) (check whether ethanol has been added before using) and mix
well with pipette. - Take out N96 Filtration Plate (H) from the negative pressure device and
put N96 Plate CB3 (H) on the device and place a waste liquid tank under
it. Transfer all liquids and precipitations of the preceding steps on N96
Plate CB3 (H), turn on the device for 5 min of suction filtration. - Add 600 μl Buffer PW to N96 Plate CB3 (H) (check whether ethanol has
been added before using). Turn on the negative pressure device for 2
min to suction filtration. - Add 600 μl Buffer PW to N96 Plate CB3 (H). Turn on the negative
pressure device for 5 min to suction filtration. - Turn off the negative pressure device and clean the waste liquid tank.
Place N96 Plate CB3 (H) at room temperature for 3min. - Place the N96 Plate CB3 (H) on the negative pressure device and place
a new N96 Well Plate under it. Add 50-200 μl Buffer TE to the middle of
membrane and incubate for 2-5 min at room temperature. Turn on the
negative pressure device for 3-5 min to suction filtration.
DP337-N96 DNAsecure Plant Kit
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
Need more info ? Contact us anytime. We’re here: Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
