DP210-TIANgel Maxi purification Kit

TIANgel Maxi Purification Kit
For DNA purification from agarose gel

TIANgel Maxi Purification Kit
(Spin Column)
Cat. no. 4992895

Storage
TIANgel Maxi DNA Purification Kit can be stored dry at room temperature
(15-30°C) for up to 15 months without showing any reduction in
performance and quality. If any precipitate forms in the buffers, it should
be dissolved by warming the buffers at 37°C for 10 min before use.

Introduction
TIANgel Maxi DNA Purification Kit combines the convenience of spincolumn technology with the selective binding properties of a uniquely
designed silica membrane and special buffer system. The kit is designed
to extract and purify DNA from any agarose gel in either TAE or TBE buffer,
meantime remove contaminants of protein, other organic compound, salts
and primers, etc. The Spin Column CA3 can purify the fragments ranging
from 100 bp to 30 kb with over 80% of recovery efficiency . Every spin
column could bind up to 20 μg DNA.
TIANgel Maxi DNA Purification Kit provides high yields of pure nucleic
acids, for direct use in applications such as restriction enzyme digestion,
PCR, sequencing, library screening, ligation, transformation, etc.

Important Notes Please read the notes before using this kit.

1. Buffer BL can improve the absorption capability ,uniformity and stability
   of the silica membrane and eliminate the effect of bad circumstance
   such as high temperature or humid on silica membrane performance.
   Check Buffers before use for salt precipitation. Dissolve any precipitate
   by warming at 37°C for several minutes.
2. Use fresh electrophoresis buffer on electrophoresis.
3. Apply TAE electrophoresis buffer if there is high demand for following
   experiment.
4. Shorten ultraviolet irradiation time when cutting agarose gel.
5. If the purification efficiency is low, check solution pH after agarose gel
   dissolved completely. If pH>7.5, add 10-30 µl of 3 M CH3
   COONa (pH 5.2)
   to the gel solution until the solution pH adjusted to 5-7.
6. Increase absorption and elution time could improve recovery efficiency
   for <100 bp and >10 kb DNA fragment.
7. The purifying efficiency is related to starting DNA quantity and elution
   volume. Insufficient starting quantity or elution volume reduces the
   recovery efficiency significantly.

DP210-TIANgel Maxi purification Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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