Blood Direct PCR Kit(Tiangen GKG204)( Go2biotech.com – Distribution for Reagents and instruments )

Rapid amplification of the target gene directly using blood as a template without extraction

Blood Direct PCR Kit(Tiangen GKG204)

Catalog number / packaging

 Manual

Storage
This kit should be stored at 2–8℃ for 3 months. For long-term storage, please keep at -20℃ . Avoid repeated freezing and thawing.

Description
This kit adopts a genetically engineered anti-inhibitor DNA polymerase to efficiently amplify single-copy genes in the human genome. The well optimized buffer system in this kit helps the polymerase strongly resist the inhibition of PCR inhibitors, thus can directly amplify DNA using blood and cultured cells as templates. This product is easy to operate and does not require complicated steps such as DNA purification or sample pretreatment.

This kit is provided as 2×MasterMix, and the reaction can be performed by simply adding the blood template and corresponding detection primers. It can be applied on the cultured cells of mammals such as humans, mice, pigs, cattle and other species, as well as fresh or 4℃ cryopreserved whole blood, anticoagulant (EDTA, citrate, heparin), liquefied blood clots and dry blood spots stored on Whatman 903 and FTA Elute commercial cards.

Features
■ Simple and fast: PCR amplification can be directly performed using blood as template, without the need for the tedious steps of sample preparation and DNA extraction.
■ High purity: The skip of sample pre-treatment and DNA extraction steps can help to avoid the cross-contamination of samples.
■ High throughput: The PCR identification for large-scale samples can be performed by combining the kit with 96/384-well PCR plates.
■ Strong universality: This kit can efficiently amplify high GC fragments or fragments with complex secondary structure, and the amplification length can be up to 5 kb.
■ Strong stress resistance: This kit can be applied for various species and blood samples preserved in different ways.

Applications
The PCR products of this kit contains “A” at the 3′-end, which can be directly used for TA vector cloning. This kit can be used for the amplification of genomic DNA fragments, high-throughput genetic analysis and genotyping (such as gene detection) analysis.

Blood Direct PCR Kit(Tiangen GKG204) Publication

• Development of a Rapid Epstein-Barr Virus Detection System Based on Recombinase Polymerase Amplification and a Lateral Flow Assay.Viruses2024-01-24 Read Article.. was extracted using a blood/cell/tissue genomic DNA extraction kit from Tiangen Biotech (Beijing; China) Co.; Ltd.;
• Composition and reagent kit for early detection of cervical high-grade lesions and cervical cancer2024-01-23 Read Article.. In this example; the blood/cell/tissue genomic DNA extraction kit (DP304) of Tiangen Biotech (Beijing) Co.; Ltd. was
• Raw Inonotus obliquus polysaccharide counteracts Alzheimer??s disease in a transgenic mouse model by activating the ubiquitin-proteosome systemNutrition Research and Practice2023-12-01 Read Article.. of 3??Tg-AD mice using the Blood/Cell/Tissue Genome DNA Extraction Kit (Tiangen; Beijing; China) according to the
• Characterization of the lncRNA-mediated ceRNA regulatory networks in preeclampsia by integrated bioinformatics.Scientific reports2023-11-01 Read Article.. mL) of each participant??the PE group and HPW group??with a Blood Cell Lysis Buffer kit (Tiangen; China).

Blood Direct PCR Kit(Tiangen GKG204)

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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