GDP419-RNAsimple Total RNA Kit

RNAsimple Total RNA Kit
For purification of high pure total RNA

RNAsimple Total RNA Kit
(Spin Column)
Cat. no. GDP419

Storage
Buffer RZ should be stored protected from light at 2-8°C for 15 months;
others stored at room temperature (15-30°C) for 15 months.

Introduction
RNAsimple Total RNA Kit is a new generation product developed by
improvement, which improves the cracking capacity of the pyrolysis
solution and the sensitivity of extraction. Meanwhile, the improvement
of the silicon matrix membrane enhances the ability of absorbing RNA,
resulting in better purity and higher quality of RNA. The kit can rapidly
extract total RNA from a variety of cells or tissues, each adsorption
column can handle 50-100 mg of tissue or 5×106
cells at a time, and can
also process a large number of different samples at the same time. The
operation was completed within an hour. The extracted RNA is free of DNA
and protein contamination and can be used in Northern Blot, Dot Blot,
PolyA Screening, in vitro transcript, RNase Protecting the Analysis, and
Molecular Cloning.

Important Note
For isolation of bacterial RNA, RNAprep Pure Cell/Bacteria Kit should be
used (Cat.no. GDP430).

Notes of preventing RNase contamination

1. Change gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-Free plastic and tips to avoid cross contamination.
3. RNA can be protected in Buffer RZ. But RNA must be stored or processed
   in RNase-Free plastic or glassware. To wipe off RNase, the glassware
   can be roasted at 150°C for 4 hours, while plastic can be dipped in 0.5
   M NaOH for 10 min, washed by RNase-Free ddH2O thoroughly, and
   sterilized.
4. Use RNase-Free ddH2O to prepare solution. (Add DEPC into water in
   clean glass container to a final concentration of 0.1% (v/v). Incubate
   overnight and autoclave for 15 min to remove any trace of DEPC.)

GDP419-RNAsimple Total RNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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