GDP430-RNAprep Pure CellBacteria Kit

RNAprep Pure Cell/Bacteria Kit
——For purification of total RNA from cultured animal cell and bacteria

RNAprep Pure Cell/Bacteria Kit(Spin Column)

Cat. no. GDP430

Compatible Reagents
Lysozyme (for bacteria RNA purification)

Storage
RNase-Free DNase I, Buffer RDD and RNase-Free ddH2O (Tubular) should
be stored at 2-8°C for 15 months; Buffer RL/β-Mercaptoethanol mix can be
stored at 2-8°C for 1 month; others stored at room temperature (15-30°C)
for 15 months.

Introduction
RNAprep Pure Cell/Bacteria Kit provides a fast, simple, and cost-effective
method for purification of total RNA from cultured animal cells and
bacteria. The purified RNA is ready for use in downstream applications
such as: RT-PCR and real-time RT-PCR, gene-chips assay, northern blot, dot
blot, polyA screening, in vitro transcript, and molecular cloning.

Notes of preventing RNase contamination

1. Change gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-Free plastic and tips to avoid cross contamination.
3. RNA can be protected in Buffer RL. But RNA must be stored or applied
   in RNase-Free plastic or glassware. To wipe off RNase, the glassware
   can be roasted at 150°C for 4 hours, while plastic can be dipped in 0.5
   M NaOH for 10 min, washed by RNase-Free ddH2O thoroughly, and
   sterilized.
4. Use RNase-Free ddH2O to confect solution.

Important points before starting

1. β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The
   final concentration of β-ME is 1%. For example, add 10 μl β-ME to 1 ml
   Buffer RL. Buffer RL containing β-ME can be stored at 2-8°C for 1 month.
   Buffer RL may form a precipitate upon storage. If necessary, re-dissolve
   by warming, and then place at room temperature (15-30°C).
2. Buffer RW is supplied as a concentrate. Before using for the first time,
   add ethanol (96-100%) as indicated on the bottle to obtain a working
   solution.
3. Perform all steps in RT if not indicated, centrifugation steps at 15-30°C
   in a standard centrifuge.

GDP430-RNAprep Pure CellBacteria Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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