GDP431-RNAprep Pure Tissue Kit

RNAprep Pure Tissue Kit
For purification of total RNA from animal tissue

RNAprep Pure Tissue Kit(Spin Column)

Cat. no. GDP431

Storage
RNase-free DNase I, Buffer RDD and RNase-free ddH2O (Tubular) should be
stored at 2-8°C for 15 months; Buffer RL/β-mercaptoethanol mix can be
stored at 2-8°C for 1 month; others stored at room temperature (15-30°C)
for 15 months.

Introduction
RNAprep Pure Tissue Kit provides a fast, simple, and cost-effective method
for purification of total RNA from animal tissues. The purified RNA is ready
for use in downstream applications such as RT-PCR, real-time RT-PCR, genechips assay, northern blot, dot blot, polyA screening, in vitro transcript,
molecular cloning and other downstream applications.

Notes of preventing RNase contamination

1. Change gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-free plastic and tips to avoid cross-contamination.
3. RNA can be protected in Buffer RL. But RNA must be stored or applied in
   RNase-free plastic or glassware. To wipe off RNase, the glassware can be
   roasted at 150°C for 4 hours, while plastic can be dipped in 0.5 M NaOH
   for 10 min, washed by RNA-free ddH2O thoroughly and sterilized.
4. Use RNase-free ddH2O to confect solution.

Important notes before starting

1. β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The
   final concentration of β-ME is 1%. For example, add 10 μl β-ME to 1 ml
   Buffer RL. Buffer RL containing β-ME can be stored at 2-8°C for 1 month.
   Buffer RL may form a precipitate upon storage. If necessary, redissolve
   by warming, and then place at room temperature (15–30°C).
2. Buffer RW is supplied as a concentrate. Before using for the first time,
   add ethanol (96–100%) as indicated on the bottle to obtain a working
   solution.
3. Perform all steps in RT if not showed.

Preparation of DNase I stock solution
Dissolve the lyophilized DNase I (1500 units) in 550 μl of the RNase-free
ddH2O. Mix gently by inverting. Do not vortex. Divide it into single-use
aliquots, and store at -30~-15°C for up to 9 months. Thawed aliquots can
be stored at 2–8°C for up to 6 weeks. Do not refreeze the aliquots after
thawing.

GDP431-RNAprep Pure Tissue Kit

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