GDP432-RNAprep Pure Plant Kit

RNAprep Pure Plant Kit
For purification of total RNA from plant cells and tissues

RNAprep Pure Plant Kit

(Spin Column)

Cat. no. GDP432

Storage
RNase-Free DNase I, Buffer RDD and RNase-Free ddH2O (Tubular) should
be stored at 2-8°C for 15 months; Buffer RL/β-mercaptoethanol mix can be
stored at 2-8°C for 1 month; others stored at room temperature (15-30°C)
for 15 months.

Introduction
RNAprep Pure Plant Kit provides a fast, simple, and cost-effective method
for purification of total RNA from plant cells and tissues. The purified
RNA is ready for use in downstream applications such as RT-PCR and realtime RT-PCR, microarray, northern blot, dot blot, polyA screening, in vitro
transcription, and molecular cloning.

Notes of preventing RNase contamination

1. Change gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-Free plastic ware and tips to avoid RNase contamination.
3. RNA can be protected in Buffer RL. But RNA must be stored or applied
   in RNase-Free plastic or glassware. To wipe off RNase, the glassware can
   be roasted at 150°C for 4 hours, while plastic can be dipped in 0.5 M
   NaOH for 10 min, washed by ddH2O thoroughly and sterilization.
4. Use RNase-Free ddH2O to confect solution

Important notes before starting

1. β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The
   final concentration of β-ME is 1%. For example, add 10 μl β-ME to 1 ml
   Buffer RL. Buffer RL containing β-ME can be stored at 2-8°C for 1 month.
   Buffer RL may form a precipitate upon storage. If necessary, redissolve
   by warming, and then place at room temperature (15-30°C).
2. RNAprep pure Plant Kit provides the lysis Buffer RL, which contain
   guanidine thiocyanate that can be used for most samples. For tissues
   with special secondary metabolites (such as milky endosperm of Maize),
   guanidine thiocyanate can lead solidification of the sample, which
   will affect RNA extraction. In this scenario, Tiangen can provide an
   alternative lysis buffer, Buffer HL, free of charge.
3. Buffer RW is supplied as a concentrate. Before using for the first time,
   add ethanol (96-100%) as indicated on the bottle to obtain a working
   solution.
4. Perform all steps and centrifugation steps at room temperature (15-30°C)
   in a standard micro centrifuge.

Preparation of DNase I stock solution
Dissolve the lyophilized DNase I (1500 U) in 550 μl RNase-Free ddH2O
(Tubular). Mix gently by inverting. Do not vortex. Divide it into single-use
aliquots, and store at -30~-15°C for up to 9 months. Thawed aliquots can
be stored at 2-8°C for up to 6 weeks. Do not refreeze the aliquots after
thawing.

GDP432-RNAprep Pure Plant Kit

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