GDP451-RNA Easy Fast TissueCell Kit

RNA Easy Fast Tissue/Cell Kit
——For purification of total RNA from animal tissue and cultured animal cell

RNA Easy Fast Tissue/Cell Kit
Cat.no. GDP451

Optional Reagents
DNase I (1500 U) (TIANGEN, Cat. no. GRT411)

Storage Conditions
Store the kit at 15-30°C for 15 months.
For the optional reagent RNase-Free DNase I, store at 2-8°C for 15 months

Introduction
The RNA Easy Fast Tissue/Cell Kit is a rapid RNA extraction kit for animal
tissues/cells developed based on the genomic DNA removal technology
exclusively developed by TIANGEN. This kit does not contain toxic reagents
such as β -mercaptoethanol or DTT, and a large number of different samples
can be processed simultaneously with the RNA extraction be completed
within 30 minutes. The total RNA extracted from this product has high yield,
good purity, an no protein or other impurities pollution. The purified RNA can
be used in RT-PCR, real time RT-PCR, chip analysis, Northern Blot, Dot Blot,
PolyA screening,in vitrotranslation, RNase protection analysis, molecular
cloning and other downstream experiments.

Notes of preventing RNase contamination

1. Change new gloves regularly. Bacteria on the skin can result in RNase
   contamination.
2. Use RNase-Free plastic products and tips to avoid cross-contamination.
3. Although RNA will not be degraded by RNase once it is in lysis buffer RLA,
   RNase-free plastics and glassware should be used for further treatment.To
   remove RNase, heat the glassware at 150°C for 4 hours, or soak the plastic
   ware in 0.5 M NaOH for 10 minutes, then washed thoroughly with water,
   and send for sterilizing.
4. Use RNase-Free ddH2
   O to prepare the solutions. (Add DEPC into water
   in clean glass container to a final concentration of 0.1% (v/v). Incubate
   overnight and autoclave for 15 min to remove any trace of DEPC.)

Important Notes

1. If the subsequent experiments have strict requirements for RNA purity,
   DNase I can be selectively added for digestion. Please refer to optional
   DNase I digestion step in the protocol. DNase I is not supplied in this
   kit, please refer to the optional reagents for the cat. no. GRT411 for
   purchasing.
2. Before the first use, absolute ethanol should be added into Buffer RW.
   Please refer to the label on the bottle for the ethanol volume.
3. If not specified, the following operations shall be carried out at room
   temperature.

GDP451-RNA Easy Fast TissueCell Kit

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