GDP441-RNAprep Pure Plant Plus Kit

RNAprep Pure Plant Plus Kit
For purification of total RNA from polysaccharides & polyphenolics-rich plant)

RNAprep Pure Plant Plus Kit
(Spin Column)
Cat. no. GDP441

Storage
RNase-Free DNase I, Buffer RDD and RNase-Free ddH2
O should be stored at 2-8°C for 15 months; Buffer SL/β-mercaptoethanol mix can be stored
at 2-8°C for 1 month; others stored at room temperature (15-30°C) for 15months.

Introduction
RNAprep Pure Plant Plus Kit provides a fast, simple, and cost-effective
method for purification of total RNA from plant cells and tissues, especially
from plant tissues rich in polysaccharides, polyphenolics and starch, such
as cotton leaves, mature rice leaves, Arabidopsis thaliana seeds, white pine
needles, banana, loquat leaves, potato tubers, apple, pear, watermelon,
kiwi fruit, Chinese rose, tobacco, sea-buckthorn, lily, etc. The purified
RNA is ready for use in downstream applications such as RT-PCR and realtime RT-PCR, microarray, northern blot, dot blot, polyA screening, in vitro
transcription, and molecular cloning.

Notes of avoiding RNase contamination

1. Wear gloves when handling RNA and all reagents, as skin is a common
   source of RNases. Change gloves frequently.
2. Use RNase-Free certified, disposable plastic ware and filter tips
   whenever possible.
3. Buffer SL could protect RNA. But for experiment, RNA should be stored
   or applied in RNase-Free plastic or glassware.
4. Use RNase-Free ddH2O to prepare solution (RNase-Free ddH2
   O: Add 0.1
   ml DEPC to 100 ml H2
   O and shake vigorously to bring DEPC into solution.
   Let the solution stand overnight. Autoclave to remove any trace of
   DEPC).

Important notes before starting

1. Add β-Mercaptoethanol (β-ME) to Buffer SL before use. The final
   concentration of β-ME is 5%. For example, add 25 μl β-ME to 475 μl
   Buffer SL. Buffer SL with β-ME can be stored at 2-8°C for up to one
   month. Buffer SL may form precipitate upon storage. If necessary, redissolve it by warming.
2. RNAprep Pure Plant Plus Kit provides a choice of Buffer HL. Buffer SL
   can be used for most plant samples. However, for tissues with special
   secondary metabolites (such as milky endosperm of maize, red bean or
   wheat seeds) or filamentous fungi, guanidine thiocyanate in Buffer SL
   may cause sample solidification, which will affect RNA extraction. In this
   condition, TIANGEN provides an alternative lysis buffer HL.
3. Buffer RW is supplied as a concentrate. Before using for the first time,
   ethanol (96-100%) should be added as indicated on the bottle to obtain
   a working solution.

Preparation of DNase I stock solution
Dissolve the lyophilized DNase I (1500U) in 550 μl RNase-Free ddH2O.
Mix gently by inverting the tube. Do not vortex. Divide it into single-use
aliquots, and store at -30~-15°C for up to 9 months. Attention: Thawed
aliquots can be stored at 2-8°C for up to 6 weeks. Do not refreeze the
aliquots after thawing.

Reagents need to be prepared by Customer
96-100% ethanol, β-Mercaptoethanol

GDP441-RNAprep Pure Plant Plus Kit

All trademarks or registered trademarks appearing on this website are the property of their respective owners.

This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

Need more info ? Contact us anytime. We’re here: Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017