ET105-Taq Plus DNA Polymerase

TaqPlus DNA Polymerase
Cat. no. 4992769/4992770
Storage: -30~-15°C for two years.
Concentration: 2.5 U/µl

Introduction
TaqPlus DNA Polymerase is a special blend of Taq
polymerase and Pfupolymerase. It possesses both 5’-3’ and 3’-5’ exonuclease activity. The advantages ofTaq Plus DNA Polymerase has high productivity and fidelity.
Comparing toTaq polymerase, Taq Plus can efficiently amplify large DNA fragments (20 Kb for simple templates and 10 Kb for complex templates). Besides, it possesses higher extension rate and amplification efficiency than PfuPolymerase.
Taq Plus DNA Polymerase generates PCR products with 3’-dA overhangs that can be directly used in TAcloning. To obtain higher cloning efficiency, however, PCR products could be purified and added 3’-dA overhangs before TA cloning procedures.

Unit Definition
One unit of TaqPlus DNA Polymerase is defined as the
amount that incorporates 10 nmol of dNTPs into acidinsoluble substrates within 30 min at 74°C with activated salmon sperm DNA as the template/primer.

Quality Control
Purity up to standard by SDS-PAGE assay; No activity of
exogenous nuclease is detected; Single gene in human
genome could be amplified effectively; No significant
activity change when stored at room temperature (15-30°C) for one week.

Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100
mM KCl, Stabilizers, 50% Glycerol

Applications
Amplify DNA fragments from complex templates (e.g.Genome) with high fidelity, for applications such as genecloning, Site-directed mutagenesis, SNP Analysis etc

ET105-Taq Plus DNA Polymerase

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