FP314-FastKing One Step RT-qPCR Kit

FastKing One Step RT-qPCR Kit (Probe)
——For real-time RT-PCR using sequencespecific, hydrolysis probes

FastKing One Step RT-qPCR Kit(Probe)
Cat. no. 4992292/4992293

Storage
FastKing One Step RT-qPCR Kit could be stored at-30~-15°Cfor 1 year.
The kit can be used with the following devices:

  1. PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System, 7500 Fast
    Real-Time PCR System, Viia 7 (Applied Biosystems)
  2. OPTICONTM / CFX96 (BIORAD)
  3. Light Cycler 480 (Roche)
  4. Smart Cycler® System (Cepheid)
  5. Mx3000P/Mx3005P (Stratagene)
  6. Other Real Time PCR thermal cycler

Introduction
FastKing One Step RT-qPCR Kit provides rapid real-time quantification using
probe method (TaqMan®, Molecular Beacon, etc). The kit allows both
reverse transcription and gene amplification to take place in a single tube,
which avoids cross contamination between samples and improves the
sensitivity of detection.
The 25×FastKing Enzyme Mix contains King reverse transcriptase, which is
a high efficient reverse transcriptase expressed by engineering bacteria;
a further-modified hot start Taq DNA polymerase, which provides high
efficiency and accuricy for the amplify reaction; and RNase inhibitor. With
a special modified hydrophobic motif, King RTase gets a significant affinity
for RNA and facilitates transcripion through of RNA templates, especially
for templates with high GC content or complex secondary structures.
The 2×FastKing One Step Probe RT-qPCR MasterMix contains appropriate
ion concentration, dNTPs and PCR enhancer. It could stabilize both
polymerasesand keep their efficiency within whole reaction process.

Materials required but not supplied

  1. Primers and probes
  2. Templates
  3. Disposable gloves and other laboratory supplies

Important Notes

  1. The RNA template should be total RNA or mRNA. We recommend using
    TIANGEN TRNzol reagent or RNAprep kit to extract high-quality RNA template.
  2. To avoid RNase contamination, the operator must: i. Put on disposable gloves
    and breathing mask. ii. Use RNase-free material such as reagents, pipette tips,
    microtubes and instruments. iii. Do the experiments in certain area that
    especially for RNA operation.
  3. 25×FastKing Enzyme Mix should be centrifuged transiently before use and
    slowly pipetted. Put it back to -30~-15°Csoon after use.
  4. Completely mix the 2×FastKing One Step Probe RT-qPCR MasterMix and
    centrifuge the reagent to the bottom of the tube before use.
  5. Use specific reverse transcription primer only. Random Primer and Oligo dT
    Primer can not be used in reverse transcription reaction.
  6. To perform several Real Time One Step RT-qPCR at the same time, a mixture of
    all reagents should be firstly prepared and then divided into each reaction tube.
    It reduces reagent loss, avoids repeatedly adding the same reagents, and
    reduces the error by adjusting the volume of each components.

FP314-FastKing One Step RT-qPCR Kit

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