morgan@go2biotech.comFP211-TIANexact Genotyping qPCR PreMix (Probe)
TIANexact Genotyping qPCR PreMix (Probe)
——For detection of SNPs by PCR (Probe method)
TIANexact Genotyping qPCR PreMix(Probe)
——Cat. No. 4992874/4992875
Storage
The TIANexact Genotyping qPCR PreMix (Probe) can be stored at -30~-15°C for one year. Itshould be stored immediately after receipt at -30~-15°C, protected from light. Thaw the 2 × TIANexact Genotyping PreMix
(Probe) and 50 × ROX Reference Dye and mix thoroughly before use. If
the 2 × TIANexact Genotyping qPCR PreMix and 50 × ROX Reference Dye
are thawed but not used, it is important to thoroughly mix prior to refreezing. The layering of salts during the thawing process and subsequent
crystallization during freezing will damage the enzyme and decrease
product performance. For frequent use, PreMix can be stored at 2-8°C for
3 months. Repeated freeze-thaw cycles should be avoided.
Introduction
TIANexact Genotyping qPCR PreMix (Probe) is a 2 × hot start PCR premix
which could be used in SNP analysis (Probe method). This premix
contains specific antibody modified TaqDNA polymerase, by which low
concentration DNA template could be easily detected. The unique buffer
system could efficiently eliminate the inhibition to PCR and the effect to
fluorescence quenching from a lot kind of PCR inhibitors. This Kit provides
an efficient and high specific method of SNP identification.
Product Features
- “Bullet-proof” genotyping—Eliminate the influence of PCR inhibitors to
SNP genotyping. - Fast reaction—Quick PCR reaction setup in most experiments.
- High fluorescent signal—Low amount of primers and probes are required
to perform SNP genotyping. - Avoid operational error—Blue indicator dye is added to monitor the
experiment setup process.
Principle of Operation
- This product can be used on qPCR and SNP analysis which uses
hydrolysis probe technique. This product eliminates the inhibition effect
of PCR inhibitors, especially for crude extract which have high stress
resistance. This product suits for the analysis of crude extracts of plants,
animals or environmental samples. - 2 × TIANexact Genotyping PreMix (Probe) contains specific antibody
modified hot start TaqDNA polymerase, by which all the enzyme
activity can be activated within 2-5 min at 95°C. It reduces the reaction
time and maximally reduces the non-specific amplification during the
PCR reaction. In addition with the optimized buffer system, this product
has high specificity and amplification efficiency on the identification of
sequence variation. - 2 × TIANexact Genotyping PreMix (Probe) includes dNTPs, enhancer and
stabilizer which increase the specificity of product and reaction sensitivity.
A blue indicator dye is also included in this product which simplifies
the experiment setup and has no negative effect on PCR reaction and
fluorescent probe. This dye could help to avoid operational error on
experiment setup of large amount of small volume samples (10 µl).
Important Notes
- Performance of reagents may reduce without mix completely. Please
gently mix the reagents by inverting the tubes and centrifuge briefly
prior to use. Do NOT vortex and avoid producing bubble. - Fluorescent probe is not included in this kit.
- Typically, best amplification results can be obtained using a primer
concentration of 300 nM and a probe concentration of 200 nM.
However, for individual determination of optimal primer concentration,
a primer titration from 100-900 nM and a probe titration from 100-250
nM can be performed. - In a 20 µl reaction volume, the amount of genomic DNA template is
usually less than 100 ng.
FP211-TIANexact Genotyping qPCR PreMix (Probe)
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
