morgan@go2biotech.comFP206-SuperReal PreMix (Probe)
SuperReal PreMix (Probe)
——For highly sensitive and specific probebased real-time PCR detection with ROX
SuperReal PreMix (Probe)
Cat. no. 4992290/4992291/4992305
Storage
SuperReal PreMix (Probe) can be stored at -30~-15°C for one year. It
should be stored immediately upon receipt at -30~-15°C. 2× SuperReal
PreMix (Probe) and 50× ROX Reference Dye should be thawed and then
mixed upside down gently to be homogenous before use. If the reagents
have been thawed but not used, it is important to thoroughly mix prior
to re-freezing. (The layering of salts during the thawing process and
subsequent crystallization during freezing will damage the enzyme and
decrease product performance.) The reagents could be stored for up to 3
months at 2-8°C if frequently used. Please avoid refreezing and thawing
repeatedly.
Introduction
SuperReal PreMix (Probe) is designed for probe based fluorescence
quantitative PCR assay. It is 2× concentrated, ready-to-use convenient mix
format.
SuperReal PreMix (Probe) provides two-component hot-start enzyme
system (Chemically modified HotStart Taq DNA Polymerase and antibody
modified Anti Taq DNA Polymerase). Combined with optimal buffer,
it shows accurate quantification, high amplification efficiency, good
repeatability and wide credibility range. We recommend SuperReal PreMix
Plus (SYBR Green) (4992214/4992215/4992248) for quantitative PCR using
SYBR Green I.
Important Notes
- The initial denaturation conditions should be 95°C 15 min to activate
the hot start enzymes. - Gently mix the reagents by inverting the tubes and centrifuge briefly
prior to use. Do NOT vortex and avoid producing bubbles. - Fluorescence probe is not included in the kit.
- 0.2 µM final concentration of probe, 0.3 µM final concentration of
primer could achieve excellent amplification result for most systems.
If need further optimization, primer concentration could be adjusted
between 0.05-0.9 µM, and probe concentration between 0.1-0.5 µM. - In a 20 µl reaction volume, the amounts of genome DNA or cDNA
template is usually less than 100 ng. If use reverse transcription product
as template, it should not comprise more than 20% of the total PCR
reaction volume.
FP206-SuperReal PreMix (Probe)
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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