morgan@go2biotech.comFP205-SuperReal PreMix Plus
SuperReal PreMix Plus (SYBR Green)
——For fast, quantitative, real-time PCR using SYBR Green
SuperReal PreMix Plus
(SYBR Green)
Cat. no. 4992214/4992215/4992248
Storage
The SuperReal PreMix Plus (SYBR Green) Kit can be stored at -30~-15°C
for one year. It should be stored immediately upon receipt at -30~-15°C,
protected from light. Thaw the 2×SuperReal PreMix Plus and 50× ROX
Reference Dye and mix thoroughly before use. If the 2×SuperReal PreMix
Plus and 50× ROX Reference Dye are thawed but not used, it is important
to thoroughly mix them prior to re-freezing. (The layering of salts during
the thawing process and subsequent crystallization during freezing will
damage the enzyme and decrease product performance.) For frequent
use, SuperReal PreMix Plus can be stored at 2-8°C for 3 months. Repeated
freeze-thaw cycles should be avoided.
Introduction
SuperReal PreMix Plus (SYBR Green) Kit is specially designed to perform
Real-time PCR in SYBR Green I fluorescent-based detection assays. The
Real-Time PCR reaction buffer, a 2× pre-mixed solution included in this
kit, provides an optimum concentration of SYBR Green I solution, which
greatly facilitates the preparation of qPCR reaction mixture. SuperReal
PreMix Plus adopts a unique dual hot-start enzymes system (chemically
modified HotStart Taq DNA polymerase and antibody modified Anti Taq
DNA Polyerase), which, plus the pre-optimized buffer solution, provides a
convenient format for highly sensitive and specific qPCR amplification.
Important Notes
- The initial denaturation conditions should be 95°C 15 min to activate
the hot start enzymes. - SuperReal PreMix Plus includes the SYBR Green I. Store the reagent in
dark and avoid direct exposition to strong light during the preparation
of PCR reaction mixtures. - Gently mix the reagents by inverting the tubes and centrifuge briefly
prior to use. Do NOT vortex and avoid producing bubble. - The purity of primers is important for the specificity of PCR. Primers
purified by PAGE or more superior methods are recommended. - Typically, best amplification results can be obtained using a primer
concentration of 0.3 µM. However, for individual determination of
optimal primer concentration, a primer titration from 0.2 µM to 0.5 μM
can be performed. - In a 20 µl reaction volume, the amount of genomic DNA or cDNA
template is usually less than 100 ng. The reverse transcription products,
if used as template, should not comprise more than 20% of the total
PCR reaction volume.
FP205-SuperReal PreMix Plus
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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