morgan@go2biotech.comET108-High Affinity HotStart Taq
High Affinity HotStart Taq
High affinity antibody-modified HotStart DNA polymerase
High Affinity HotStart Taq
Cat. No. 4992773/4992774
Storage Conditions
The product can be stored at -30~-15°C for one year.Please store the
product at -30~-15°Cimmediately after receiving it. The product can
be kept for 1 year at -30~-15°C. When taken out from -30~-15°C, thaw
the frozen 10 × HA buffer and 5 x Probe qPCR Buffer, then shake upside
down slightly to mix. Apply the solutions when the solution is completely
homogeneous. If the buffers are used frequently for a period of time, they
can be stored at 2-8°Cfor 3 months. Repeated freezing and thawing should
be avoided.
Product Description
The High Affinity HotStart Taqin this product is a mixture of TIANGEN
TaqDNA Polymerase and a monoclonal antibody to TaqDNA polymerase,
which is suitable for the HotStart PCR experiment. Taqmonoclonal
antibody will combine with Taqpolymerase and inhibit its polymerase
activity before the PCR temperature is elevated. The high affinity
antibody in this product can completely inhibit the enzyme activity of Taq
polymerase under normal temperature to keep the whole reaction system
with high specificity. In addition, the TaqDNA polymerase in this product
has high template affinity, which can improve the efficiency and specificity
of amplification. The 10 × HA Buffer is a specially optimized PCR reaction
buffer for this polymerase, which can guarantee the best performance
of the enzyme. The 5 × Probe qPCR Buffer is a reaction additive specially
optimized for the probe qPCR, which can be used in the probe qPCR
reaction in combined with the 10 × HA Buffer, ensuring a wider application
of the product. In addition, the 3′-end of the PCR product produced by the
kit is A, which can be cloned directly with TA vector.
Product Highlights
- High affinity system: High Affinity HotStart Taqis a specific antibody
modified hot start DNA polymerase with high antibody affinity. The
TaqDNA polymerase has high template affinity, stable amplification
efficiency and high specificity, and the well-designed buffer system also
makes the PCR reaction with high sensitivity. - Strong stability: For complex templates, low copy templates, multiplex
PCR reaction and other experiments that ordinary DNA polymerase
cannot perform, but this product has a high reaction success rate. - Wide application: This product is not only suitable for general PCR
analysis, but also for quantitative PCR analysis.
Precautions
- There is no need to add the 5 × Probe qPCR Buffer in general PCR and
dye-based quantitative PCR, but only in probe quantitative PCR. - In probe quantitative PCR, 250 nM final concentration of primers and
200 nM final concentration of probe can lead to good amplification
results in most systems. If the primer concentration needs to be further
optimized, it can be adjusted in the range of 50-900 nM. If the probe
concentration needs to be remove several nucleotides from 5’ or 3’
terminal.
Definition of activity
The activity of 1 unit (U) High Affinity HotStart Taqis defined as the
amount of enzyme required to mix 10 nmol of deoxynucleotides into acid
insoluble substrates in 3 minutes at 74°Cwith the active sperm DNA of
salmon as template/primers.
Quality Control
Purity up to standard by SDS-PAGE assay; No activity of exogenous nuclease
is detected; Single gene in human genome could be amplified effectively;
No significant activity change when stored at room temperature for one
week.
Applications
The product is suitable for conventional PCR reaction, complex template,
low copy template amplification, multiplex PCR and quantitative PCR, etc.
ET108-High Affinity HotStart Taq
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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