GDP761-Magnetic Tissue Cell Blood Total RNA Kit

Magnetic Tissue/Cell/Blood Total RNA Kit

Magnetic Tissue/Cell/Blood Total RNA Kit
Cat.no. GDP761

Optional reagents and tools
DNase I (Cat.no. GRT411); Magnetic Stand (Cat.no. OSE-MF-01); 10×Red
cell Lysis Buffer H; RNase-Free Columns CS set.

Storage
This kit can be stored at room temperature (15-30°C) under dry condition
for 15 months.

Introduction
The kit adopts magnetic beads with unique separation function and a
unique buffer system to separate and purify high-quality total RNA from
various tissues, cells and blood. The unique embedded magnetic beads
have strong affinity for nucleic acid under certain conditions, and when the
conditions change, the magnetic beads will release adsorbed nucleic acid,
thus achieving the purpose of fast separation and purification of nucleic
acid.
The product can be perfectly matched with Kingfisher Flex96 and TGuide
S32 Automated Nucleic Acid Extractors. Magnetic beads are adsorbed,
transferred and released by special magnetic rods, thus realizing the
transfer of magnetic beads and nucleic acids. The whole experimental
process is safe and convenient, and the extracted total RNA has high purity
and is free from contamination of genomes, proteins and other impurities.
If high-throughput automated extraction is required, please contact
TIANGEN for the integration solution.
RNA purified by the kit is suitable for various downstream experiments
such as RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot,
PolyA screening, in vitrotranslation, RNase protection analysis, molecular
cloning, etc.

Features

1. The kit can meet the requirements of manual extraction as well as batch
   extraction on various high-throughput platforms.
2. The products obtained by the kit meet the requirements of downstream
   detection experiments and NGS analysis.

Notes Please read these notes before using this kit.

1. Before operation, add β-mercaptoethanol to Buffer RL to a final
   concentration of 1%, for example, add 10 μl of β-mercaptoethanol to
   1 ml of Buffer RL. This buffer is best prepared right before use. The
   prepared Buffer RL can be stored at 2-8°C for 1 month. The Buffer RL
   may form precipitates during storage, and if any precipitates appear,
   please heat and dissolve before use.
2. Add 96-100% ethanol to Buffer RD and Buffer RW before the first use.
   Please refer to the label on the bottle for the added volume.

Self-provided reagents and instruments
β-mercaptoethanol, isopropanol, 96-100% ethanol, homogenization
equipment (mortar, electric homogenizer, etc.), magnetic stand.

GDP761-Magnetic Tissue Cell Blood Total RNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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